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一种常规鉴定厌氧菌的方法,特别是非芽孢形成菌(作者译)

[A method for routine identification of anaerobic bacteria, in particular of non-sporogenic forms (author's transl)].

作者信息

Lodenkämper H, Rühlcke U

出版信息

Zentralbl Bakteriol Orig A. 1975 Aug;232(4):554-63.

PMID:1106065
Abstract

In the Institute of Bacteriology of Allgemeines Krankenhaus St. Georg, Hamburg, all clinical specimens received (except stools, sputum, and vaginal swabs) have been studied for the presence of anaerobians since 1947. More than 25 years' experience has convinced the authors that not infrequently, non-sporogenic anaerobians occur as infectious agents. Statistical data compiled for the period 1956-1972 revealed that from a total of 71,973 specimens contaminated with bacteria, 7,047 (9.8%) were containing non-sporogenic anaerobians. Pure anaerobic cultures were obtained in 2.404 cases (3.3%) and mixed cultures of aerobic and anaerobic bacteria in 4,643 cases (6.5%). The pathogenic importance was revealed by identifications from osteomyelitis cases: 11-13.7% pure cultures and 17% mixed cultures (with aerobians). Also in cases of total endoprosthesis of complicated course they were detected in up to 23%. On account of the frequent cultures of non-sporogenic anaerobians obtained from clinical specimens, the authors demand a routine analysis of all clinical specimens for the presence of anerobians...

摘要

自1947年以来,汉堡圣乔治综合医院细菌学研究所对所接收的所有临床标本(粪便、痰液和阴道拭子除外)进行了厌氧菌检测。25年多的经验使作者确信,非产芽孢厌氧菌作为感染源的情况并不罕见。1956年至1972年期间汇编的统计数据显示,在总共71973份被细菌污染的标本中,有7047份(9.8%)含有非产芽孢厌氧菌。2404例(3.3%)获得了纯厌氧培养物,4643例(6.5%)获得了需氧菌和厌氧菌的混合培养物。骨髓炎病例的鉴定显示了其致病重要性:11%-13.7%为纯培养物,17%为混合培养物(与需氧菌混合)。在复杂病程的全关节置换病例中,它们的检出率高达23%。由于从临床标本中经常培养出非产芽孢厌氧菌,作者要求对所有临床标本进行常规分析,以检测厌氧菌的存在……

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