Essers L, Haralambie E
Zentralbl Bakteriol Orig A. 1977 Jul;238(3):394-401.
The API 20A System was tested in three modifications: a) The microtubes were inoculated with the API anaerobe basal medium, filled up completely with sterile mineral oil and incubated aerobically. b) The test strips were inoculated with the basal medium and incubated in an anaerobic chamber. c) The strips were inoculated with a modified Viande-Levure medium containing Tween 80, vitamin K3 and hemin. The microtubes were covered with sterile mineral oil and incubated in an anaerobic chamber. Each procedure was compared with the conventional method (PRAS) of the Virginia Polytechnic Institute. The overall agreement between the three modifications of the API System and the conventional method was 83.2, 91.7, and 98.5% related to the number of tests performed. The advantage of the modified medium was also demonstrated by measuring the growth rate of some anaerobes in thioglycolate broth, API basal medium and VL-medium, modified as mentioned above, nephelometrically. So the micromethod is more accurate and reliable when inoculated with an improved medium.
对API 20A系统进行了三种改进测试：a）将API厌氧菌基础培养基接种到微量管中，完全充满无菌矿物油并进行需氧培养。b）将基础培养基接种到测试条上，并在厌氧箱中培养。c）将含有吐温80、维生素K3和血红素的改良Viande-Levure培养基接种到测试条上。微量管用无菌矿物油覆盖，并在厌氧箱中培养。将每个步骤与弗吉尼亚理工学院的传统方法（PRAS）进行比较。API系统的三种改进方法与传统方法之间的总体一致性分别为83.2%、91.7%和98.5%，这与所进行的测试数量有关。通过比浊法测量某些厌氧菌在硫乙醇酸盐肉汤、API基础培养基和上述改良的VL培养基中的生长速率，也证明了改良培养基的优势。因此，当接种改良培养基时，微量法更准确可靠。
