Fujita Y, Kojima K, Kubo S
Scientific Investigation Research Laboratory, Tokushima Prefectural Police Headquarters, Japan.
Nihon Hoigaku Zasshi. 2000 Aug;54(2):227-32.
Identification of human blood is very important in the practice of criminal investigation. Methods that are species-specific and highly sensitive usually require special laboratory equipment. To develop a method that is specific, sensitive, and convenient for use at the crime scene, we applied a sandwich-hybridization method for human blood identification. The test kit, which uses anti-human hemoglobin (Hb) monoclonal antibody, showed high species specificity and could detect as little as 20 ng human Hb. Cross-reactivity was observed only to baboon. It was able to detect dilutions up to 5,000,000 times and to identify a 15.5-year-old human bloodstain. Because the method is rapid (2 minutes) and does not require any special equipment, it is considered useful for crime scene investigation.
在刑事侦查实践中，鉴定人类血液非常重要。具有物种特异性且高度灵敏的方法通常需要特殊的实验室设备。为开发一种特异性强、灵敏度高且便于在犯罪现场使用的方法，我们应用了夹心杂交法进行人类血液鉴定。该检测试剂盒使用抗人血红蛋白（Hb）单克隆抗体，具有高度的物种特异性，能够检测低至20纳克的人血红蛋白。仅观察到与狒狒有交叉反应。它能够检测高达500万倍的稀释液，并能鉴定出15.5年前的人类血迹。由于该方法快速（2分钟）且不需要任何特殊设备，因此被认为对犯罪现场调查很有用。
