Murakami M, Hoshikawa Y, Satoh Y, Ito H, Tajima M, Okinaga K, Miyazawa Y, Kurata T, Sairenji T
Department of Biosignaling, Tottori University, Yonago, 683-8503, USA.
Virology. 2000 Nov 10;277(1):20-6. doi: 10.1006/viro.2000.0602.
To study the tumorigenesis of Epstein-Barr virus (EBV)-positive epithelial cell lines GT38 and GT39 derived from human gastric tissues, we inoculated these cells under the skin of severe combined immunodeficient (SCID) mice. The development of tumors was observed in each of the mice about 2 months after the inoculation. The tumors were diagnosed with undifferentiated carcinoma by hematoxylin/eosin staining. EBV-encoded small RNA1 was detected in the paraffin-embedded tumor sections. The tumor cells had human chromosome. The circular, but not linear, EBV DNA was detected in the tumors. The molecular sizes of EBV DNA termini were the same as that of the inoculated GT38 or GT39 cells. The expressions of EBV nuclear antigen 2 and latent membrane protein 1 reduced in the tumors. Transcripts of BamHI C and W promoters in latency III were detected in the tumors and the cultured cells in vitro. The tumor cells were passaged from one SCID mouse to other SCID mice and to cultures in vitro. This is the first evidence that the EBV-positive epithelial cell lines produced tumors in the SCID mouse.
为了研究源自人胃组织的爱泼斯坦-巴尔病毒（EBV）阳性上皮细胞系GT38和GT39的肿瘤发生情况，我们将这些细胞接种到严重联合免疫缺陷（SCID）小鼠的皮下。接种后约2个月，在每只小鼠中均观察到肿瘤的形成。通过苏木精/伊红染色将肿瘤诊断为未分化癌。在石蜡包埋的肿瘤切片中检测到EBV编码的小RNA1。肿瘤细胞具有人类染色体。在肿瘤中检测到环状而非线性的EBV DNA。EBV DNA末端的分子大小与接种的GT38或GT39细胞相同。肿瘤中EBV核抗原2和潜伏膜蛋白1的表达降低。在肿瘤和体外培养的细胞中检测到潜伏期III中BamHI C和W启动子的转录本。肿瘤细胞从一只SCID小鼠传代至其他SCID小鼠并进行体外培养。这是EBV阳性上皮细胞系在SCID小鼠中产生肿瘤的首个证据。
