Cusi M G, Zurbriggen R, Valassina M, Bianchi S, Durrer P, Valensin P E, Donati M, Glück R
Department of Molecular Biology, Section of Microbiology, University of Siena, Via Laterina 8, Siena, 53100, Italy.
Virology. 2000 Nov 10;277(1):111-8. doi: 10.1006/viro.2000.0605.
To improve the efficiency of liposome-mediated DNA transfer as a tool for gene therapy or vaccinology, we have further developed a new delivery system based on the modified immunopotentiating reconstituted influenza virus (IRIV). In this study, we engineered a plasmid DNA vector expressing the mumps virus hemagglutinin or the fusion protein. The administration of this DNA vaccine delivered by influenza virosomes, in combination with the mucosal adjuvant Escheriagen via the intranasal route, was efficient for inducing an immune response, both mucosally and systemically, in mice. The production of IgG2a mumps virus-specific antibodies and the secretion of interleukin 10 (IL-10) by antigen-specific T cells indicated that not only Th1 but also Th2 responses were induced by this DNA vaccine formulation. These results suggest that cationic virosomes in combination with Escheriagen may have great potential as an efficient delivery system for intranasal DNA immunization and provide an immune barrier at the mucosal sites.
为提高脂质体介导的DNA转移作为基因治疗或疫苗学工具的效率，我们进一步开发了一种基于修饰的免疫增强重组流感病毒（IRIV）的新型递送系统。在本研究中，我们构建了一种表达腮腺炎病毒血凝素或融合蛋白的质粒DNA载体。通过鼻内途径，由流感病毒体递送的这种DNA疫苗与粘膜佐剂大肠杆菌素联合给药，在小鼠体内诱导粘膜和全身免疫反应方面是有效的。IgG2a腮腺炎病毒特异性抗体的产生以及抗原特异性T细胞分泌白细胞介素10（IL-10）表明，这种DNA疫苗制剂不仅诱导了Th1反应，还诱导了Th2反应。这些结果表明，阳离子病毒体与大肠杆菌素联合使用可能作为鼻内DNA免疫的有效递送系统具有巨大潜力，并在粘膜部位提供免疫屏障。
