一种激活或抑制转录的基础转录因子。

A basal transcription factor that activates or represses transcription.

作者信息

Willy P J, Kobayashi R, Kadonaga J T

机构信息

Section of Molecular Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093-0347, USA.

出版信息

Science. 2000 Nov 3;290(5493):982-5. doi: 10.1126/science.290.5493.982.

DOI:10.1126/science.290.5493.982

PMID:11062130

Abstract

We have identified an activity that is required for transcription of downstream promoter element (DPE)-containing core promoters in vitro. The purified factor was found to be the Drosophila homolog of the transcriptional repressor known as NC2 or Dr1-Drap1. Purified recombinant dNC2 activates DPE-driven promoters and represses TATA-driven promoters. A mutant version of dNC2 can activate DPE promoters but is unable to repress TATA promoters. Thus, the activation and repression functions are distinct. These studies reveal that NC2 (Dr1-Drap1) is a bifunctional basal transcription factor that differentially regulates gene transcription through DPE or TATA box motifs.

摘要

我们已经鉴定出一种在体外转录含下游启动子元件（DPE）的核心启动子所需的活性。发现纯化的因子是被称为NC2或Dr1-Drap1的转录抑制因子的果蝇同源物。纯化的重组dNC2激活DPE驱动的启动子并抑制TATA驱动的启动子。dNC2的突变体版本可以激活DPE启动子，但不能抑制TATA启动子。因此，激活和抑制功能是不同的。这些研究表明，NC2（Dr1-Drap1）是一种双功能基础转录因子，通过DPE或TATA盒基序差异调节基因转录。