Hsu Jer-Yuan, Juven-Gershon Tamar, Marr Michael T, Wright Kevin J, Tjian Robert, Kadonaga James T
Section of Molecular Biology, University of California at San Diego, La Jolla, California 92093, USA.
Genes Dev. 2008 Sep 1;22(17):2353-8. doi: 10.1101/gad.1681808. Epub 2008 Aug 14.
The RNA polymerase II core promoter is a structurally and functionally diverse transcriptional module. RNAi depletion and overexpression experiments revealed a genetic circuit that controls the balance of transcription from two core promoter motifs, the TATA box and the downstream core promoter element (DPE). In this circuit, TBP activates TATA-dependent transcription and represses DPE-dependent transcription, whereas Mot1 and NC2 block TBP function and thus repress TATA-dependent transcription and activate DPE-dependent transcription. This regulatory circuit is likely to be one means by which biological networks can transmit transcriptional signals, such as those from DPE-specific and TATA-specific enhancers, via distinct pathways.
RNA聚合酶II核心启动子是一个结构和功能多样的转录模块。RNA干扰缺失和过表达实验揭示了一个遗传回路，该回路控制着来自两个核心启动子基序（TATA框和下游核心启动子元件DPE）的转录平衡。在这个回路中，TBP激活依赖TATA的转录并抑制依赖DPE的转录，而Mot1和NC2则阻断TBP的功能，从而抑制依赖TATA的转录并激活依赖DPE的转录。这种调节回路可能是生物网络通过不同途径传递转录信号（如来自DPE特异性和TATA特异性增强子的信号）的一种方式。
