给予CIDR - B、前列腺素和雌二醇或促性腺激素释放激素的肉牛的发情同步化和妊娠率

Estrus synchronization and pregnancy rates in beef cattle given CIDR-B, prostaglandin and estradiol, or GnRH.

作者信息

Martínez M F, Kastelic J P, Adams G P, Janzen E, McCartney D H, Mapletoft R J

机构信息

Department of Herd Medicine and Theriogenology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon.

出版信息

Can Vet J. 2000 Oct;41(10):786-90.

PMID:11062836

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1476379/

Abstract

Two experiments were conducted to determine estrous response and pregnancy rate in beef cattle given a controlled internal drug release (CIDR-B) device plus prostaglandin F2 alpha (PGF) at CIDR-B removal, and estradiol or gonadotropin releasing hormone (GnRH). In Experiment I, crossbred beef heifers received a CIDR-B device and 1 mg estradiol benzoate (EB), plus 100 mg progesterone (E + P group; n = 41), 100 micrograms gonadotropin releasing hormone (GnRH group; n = 42), or no further treatment (Control group; n = 42), on Day 0. On Day 7, CIDR-B devices were removed and heifers were treated with PGF. Heifers in the E + P group were given 1 mg EB, 24 h after PGF, and then inseminated 30 h later. Heifers in the GnRH group were given 100 micrograms GnRH, 54 h after PGF, and concurrently inseminated. Control heifers were inseminated 12 h after onset of estrus. The estrous rate was lower (P < 0.01) in the GnRH group (55%) than in either the E + P (100%) or Control (83%) groups. The mean interval from CIDR-B removal to estrus was shorter (P < 0.01) and less variable (P < 0.01) in the E + P group than in the GnRH or Control groups. Pregnancy rate in the E + P group (76%) was higher (P < 0.01) than in the GnRH (48%) or Control (38%) groups. In Experiment II, 84 cows were treated similarly to the E + P group in Experiment I. Cows received 100 mg progesterone and either 1 mg EB or 5 mg estradiol-17 beta (E-17 beta) on Day 0 and either 1 mg of EB or 1 mg of E-17 beta on Day 8 (24 h after CIDR-B removal), in a 2 x 2 factorial design, and were inseminated 30 h later. There were no differences among groups for estrous rates or conception rates. The mean interval from CIDR-B removal to estrus was 44.2 h, s = 11.2. Conception rates were 67%, 62%, 52%, and 71% in Groups E-17 beta/E-17 beta, E-17 beta/EB, EB/E-17 beta, and EB/EB, respectively. In cattle given a CIDR-B device and estradiol plus progesterone, treatment with either EB or E-17 beta effectively synchronized estrus and resulted in acceptable conception rates to fixed-time artificial insemination.

摘要

进行了两项试验，以确定在取出控制内部药物释放（CIDR - B）装置时给予前列腺素F2α（PGF）以及雌二醇或促性腺激素释放激素（GnRH）的肉牛的发情反应和妊娠率。在试验I中，杂交肉牛小母牛在第0天接受CIDR - B装置和1毫克苯甲酸雌二醇（EB），加100毫克孕酮（E + P组；n = 41）、100微克促性腺激素释放激素（GnRH组；n = 四十二），或不进行进一步处理（对照组；n = 42）。在第7天，取出CIDR - B装置，并用PGF处理小母牛。E + P组的小母牛在PGF处理后24小时给予1毫克EB，然后在30小时后进行授精。GnRH组的小母牛在PGF处理后54小时给予100微克GnRH，并同时进行授精。对照小母牛在发情开始后12小时进行授精。GnRH组的发情率（55%）低于E + P组（100%）或对照组（83%）（P < 0.01）。E + P组从取出CIDR - B装置到发情的平均间隔时间比GnRH组或对照组短（P < 0.01）且变异性小（P < 0.01）。E + P组的妊娠率（76%）高于GnRH组（48%）或对照组（38%）（P < 0.01）。在试验II中，84头母牛的处理方式与试验I中的E + P组相似。母牛在第0天接受100毫克孕酮和1毫克EB或5毫克17β - 雌二醇（E - 17β），并在第8天（取出CIDR - B装置后24小时）接受1毫克EB或1毫克E - 17β，采用2×2析因设计，并在30小时后进行授精。各组之间的发情率或受孕率没有差异。从取出CIDR - B装置到发情的平均间隔时间为44.2小时，s = 11.2。E - 17β/E - 17β组、E - 17β/EB组、EB/E - 17β组和EB/EB组的受孕率分别为67%、62%、52%和71%。在给予CIDR - B装置和雌二醇加孕酮的牛中，用EB或E - 17β处理可有效同步发情，并使定时人工授精的受孕率达到可接受水平。