Samir Haney, El-Sherbiny Hossam R, Ahmed Ahmed Ezzat, Ahmad Sindi Ramya, Al Syaad Khalid M, Abdelnaby Elshymaa A
Theriogenology Department, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
Biology Department, College of Science, King Khalid University, Abha 61413, Saudi Arabia.
Animals (Basel). 2023 Jul 18;13(14):2340. doi: 10.3390/ani13142340.
The postpartum (PP) period is a crucial stage for the resumption of reproductive performance and ovarian cyclicity in dairy buffaloes. The present study aimed, for the first time, to assess the effect of the administration of estradiol benzoate (EB) on ovarian and uterine hemodynamics in PP dairy buffaloes. Eight pluriparous acyclic domestic buffaloes were enrolled in the present experiment and received a dose of 10 mg of estradiol benzoate (EB) intramuscularly 4 weeks after parturition. All animals were examined two times before EB administration (days -3, and -1) and on the day of EB administration (day 0), followed by examinations on days 1, 3, 5, 7, and 9 post-EB administration. The middle uterine artery (MUA) and ovarian artery (OA) blood flow patterns were assessed using a color Doppler ultrasound device. The reproductive parameters were (1) the cross-sectional diameters (cm) of the OA and MUA, (2) cranial uterine horn thickness (UHT; cm), and (3) hemodynamic changes within the MUA on both the ipsi- and contra-lateral sides of the previous pregnant horn and within the OA corresponding to the ovarian tissues. The examined blood flow parameters were the pulsatility index (PI), resistance index (RI), peak systolic/end-diastolic ratio (S/D), time-averaged maximum velocity (TAV; cm/s), uterine blood flow rate (BFR; bpm), and uterine blood flow volume (BFV; mL/min). Concomitantly, blood samples were collected from the coccygeal vein, and the sera were stored at -18 °C for use in estradiol (E2-17β) and nitric oxide (NO) assays. The results revealed increases in both OA and MUA cross-sectional diameter (cm) on the ipsi-lateral and contra-lateral ( < 0.05) sides within 24 h until day 9 post-treatment. The values of the RI and PI of blood flow within the OA and MUA on the ipsi-lateral and contra-lateral sides of the previous pregnancy were obviously lower ( < 0.05) at 24 h after the administration of EB, and then, started to gradually elevate, reaching the pre-treatment values on day 9 after EB administration. Both the BFR and BFV in the OA and MUA significantly increased from 24 h to 72 h after EB administration on both the ipsi-lateral and contra-lateral sides ( < 0.05); then, their values started to decrease to reach the pretreatment value on day 9 after EB administration. Both E2 and NO concentrations significantly increased ( < 0.05) from 24 h until day 3 after EB injection and then started to decline after that, reaching the pre-treatment value on day 9. In conclusion, the administration of EB enhances the ovarian and uterine blood flow concomitantly with increased levels of NO in PP dairy buffaloes.
产后（PP）期是奶水牛恢复繁殖性能和卵巢周期性的关键阶段。本研究首次旨在评估苯甲酸雌二醇（EB）给药对产后奶水牛卵巢和子宫血流动力学的影响。本实验纳入了8头经产无发情周期的家养水牛，在分娩后4周肌肉注射10 mg苯甲酸雌二醇（EB）。在EB给药前（-3天和-1天）和给药当天（0天）对所有动物进行两次检查，随后在EB给药后第1、3、5、7和9天进行检查。使用彩色多普勒超声设备评估子宫中动脉（MUA）和卵巢动脉（OA）的血流模式。生殖参数包括：（1）OA和MUA的横截面积（cm）；（2）子宫角前壁厚度（UHT；cm）；（3）先前怀孕侧子宫角同侧和对侧MUA以及与卵巢组织相对应的OA内的血流动力学变化。所检查的血流参数包括搏动指数（PI）、阻力指数（RI）、收缩期峰值/舒张末期比值（S/D）、时间平均最大流速（TAV；cm/s）、子宫血流速率（BFR；bpm）和子宫血流量（BFV；mL/min）。同时，从尾静脉采集血样，血清储存于-18°C用于雌二醇（E2-17β）和一氧化氮（NO）检测。结果显示，治疗后至第9天，同侧和对侧的OA和MUA横截面积（cm）在24小时内均增加（<0.05）。给药后24小时，先前怀孕侧同侧和对侧OA和MUA内血流的RI和PI值明显较低（<0.05），然后开始逐渐升高，在EB给药后第9天达到治疗前值。给药后24小时至72小时，同侧和对侧OA和MUA的BFR和BFV均显著增加（<0.05）；然后，其值开始下降，在EB给药后第9天达到治疗前值。EB注射后24小时至第3天，E2和NO浓度均显著增加（<0.05），之后开始下降，在第9天达到治疗前值。总之，在产后奶水牛中，EB给药可增强卵巢和子宫血流，同时NO水平升高。
