Lambert P A, Worthington T, Tebbs S E, Elliott T S
Pharmaceutical and Biological Sciences, Aston University, Birmingham B4 7ET, UK.
FEMS Immunol Med Microbiol. 2000 Nov;29(3):195-202. doi: 10.1111/j.1574-695X.2000.tb01523.x.
We describe the characterisation of a novel glycerophosphoglycolipid (termed lipid S) produced by Staphylococcus epidermidis grown in a chemically defined medium. Lipid S is a short chain length form of the cellular lipoteichoic acid (LTA). It shares common antigenic determinants with LTA, but its chain length of six glycerophosphate units contrasts with 40-42 units in LTA. Lipid S is exocellular and can be recovered from liquid growth medium whereas LTA is associated with the cell wall and membrane. Healthy individuals have low serum levels of IgG against lipid S, but significantly higher titres have been detected in serum from patients with central venous catheter-related sepsis due to coagulase-negative staphylococci and infection of orthopaedic prostheses. An indirect enzyme-linked immunosorbent assay test based on lipid S allows the rapid diagnosis of Gram-positive infection and may have clinical applications in the management of patients with sepsis.
我们描述了在化学限定培养基中生长的表皮葡萄球菌产生的一种新型甘油磷酸糖脂（称为脂质S）的特性。脂质S是细胞脂磷壁酸（LTA）的短链形式。它与LTA具有共同的抗原决定簇，但其六个甘油磷酸单元的链长与LTA中40 - 42个单元形成对比。脂质S是胞外的，可从液体生长培养基中回收，而LTA与细胞壁和细胞膜相关。健康个体血清中抗脂质S的IgG水平较低，但在因凝固酶阴性葡萄球菌导致的中心静脉导管相关败血症患者和骨科假体感染患者的血清中检测到的滴度明显更高。基于脂质S的间接酶联免疫吸附测定试验可快速诊断革兰氏阳性感染，可能在脓毒症患者的管理中具有临床应用价值。
