Worthington T, Lambert P A, Traube A, Elliott T S J
Department of Clinical Microbiology, University Hospital, Edgbaston, Birmingham B15 2TH, UK.
J Clin Pathol. 2002 Jan;55(1):41-3. doi: 10.1136/jcp.55.1.41.
To develop and evaluate a rapid enzyme linked immunosorbent assay (ELISA) for the diagnosis of intravascular catheter related sepsis caused by coagulase negative staphylococci.
Forty patients with a clinical and microbiological diagnosis of intravascular catheter related sepsis and positive blood cultures, caused by coagulase negative staphylococci, and 40 control patients requiring a central venous catheter as part of their clinical management were recruited into the study. Serum IgG responses to a previously undetected exocellular antigen produced by coagulase negative staphylococci, termed lipid S, were determined in the patient groups by a rapid ELISA.
There was a significant difference (p = < 0.0001) in serum IgG to lipid S between patients with catheter related sepsis and controls. The mean antibody titre in patients with sepsis caused by coagulase negative staphylococci was 10 429 (range, no detectable serum IgG antibody to 99 939), whereas serum IgG was not detected in the control group of patients.
The rapid ELISA offers a simple, economical, and rapid diagnostic test for suspected intravascular catheter related sepsis caused by coagulase negative staphylococci, which can be difficult to diagnose clinically. This may facilitate treatment with appropriate antimicrobials and may help prevent the unnecessary removal of intravascular catheters.
开发并评估一种快速酶联免疫吸附测定法（ELISA），用于诊断凝固酶阴性葡萄球菌引起的血管内导管相关败血症。
招募了40例临床和微生物学诊断为血管内导管相关败血症且血培养阳性、由凝固酶阴性葡萄球菌引起的患者，以及40例作为临床治疗一部分需要中心静脉导管的对照患者进入研究。通过快速ELISA法测定患者组中针对一种先前未被检测到的由凝固酶阴性葡萄球菌产生的细胞外抗原（称为脂质S）的血清IgG反应。
导管相关败血症患者与对照患者之间针对脂质S的血清IgG存在显著差异（p = < 0.0001）。由凝固酶阴性葡萄球菌引起败血症的患者中抗体平均滴度为10429（范围，从无可检测到的血清IgG抗体至99939），而对照组患者未检测到血清IgG。
快速ELISA为疑似由凝固酶阴性葡萄球菌引起的血管内导管相关败血症提供了一种简单、经济且快速的诊断测试，临床上这种败血症可能难以诊断。这可能有助于使用适当的抗菌药物进行治疗，并有助于防止不必要地拔除血管内导管。
