Dooley P C, Long B M, West J M
La Trobe University, School of Human Biosciences, Bundoora, 3083, Victoria, Australia.
Comp Biochem Physiol A Mol Integr Physiol. 2000 Oct;127(2):155-65. doi: 10.1016/s1095-6433(00)00247-6.
The concentrations of free amino acids were measured in whole claw muscle, single fibres and haemolymph of Australian freshwater crayfish, Cherax destructor, during the intermoult stage. The average total pool of amino acids in short-sarcomere fibres (179 mmol kg(-1)) was 60% greater than in long-sarcomere fibres, due to higher concentrations of alanine, cysteine, glutamate, leucine and proline. The two fibre types exhibited differences in the banding pattern of the isoforms of troponin using gel electrophoresis. The average pool of amino acids in haemolymph was 2.7 mmol kg(-1). Cherax has symmetrical claws and the total pool of amino acids from whole muscles (approx. 79 mmol kg(-1)) was similar in left and right claw muscles. In animals acclimated to osmotic environments between 0 and 220 mOsm, the osmotic pressure of the haemolymph increased from 356 to 496 mOsm, but no systematic changes were observed in the amino acid profiles of muscles or haemolymph. The major findings were that (a) concentrations of amino acids differed between the two major fibre types in claw muscle and (b) amino acids in the muscle fibres did not play a major part in intracellular osmoregulation in Cherax, suggesting this species is an anisosmotic regulator.
在蜕壳间期，对澳大利亚淡水小龙虾（Cherax destructor）的整个爪肌、单根肌纤维和血淋巴中的游离氨基酸浓度进行了测量。短肌节纤维中氨基酸的平均总库（179 mmol kg⁻¹）比长肌节纤维中的高60%，这是由于丙氨酸、半胱氨酸、谷氨酸、亮氨酸和脯氨酸的浓度较高。使用凝胶电泳分析，这两种纤维类型在肌钙蛋白同工型的条带模式上存在差异。血淋巴中氨基酸的平均库为2.7 mmol kg⁻¹。Cherax具有对称的爪子，左右爪肌中全肌氨基酸的总库（约79 mmol kg⁻¹）相似。在适应0至220 mOsm渗透环境的动物中，血淋巴的渗透压从356 mOsm增加到496 mOsm，但在肌肉或血淋巴的氨基酸谱中未观察到系统性变化。主要发现为：（a）爪肌中两种主要纤维类型的氨基酸浓度不同；（b）肌纤维中的氨基酸在Cherax的细胞内渗透调节中不起主要作用，这表明该物种是一种异渗调节者。
