外周血中播散癌细胞的分子检测及结肠癌中唾液酸化抗原的表达

Molecular detection of disseminated cancer cells in the peripheral blood and expression of sialylated antigens in colon cancers.

作者信息

Ichikawa D, Kitamura K, Tani N, Nishida S, Tsurutome H, Hakomori S, Ikeda E, Mutoh F, Kurioka H, Yamagishi H

机构信息

Department of Surgery, Kyoto First Red Cross Hospital, Kyoto, Japan.

出版信息

J Surg Oncol. 2000 Oct;75(2):98-102. doi: 10.1002/1096-9098(200010)75:2<98::aid-jso5>3.0.co;2-r.

DOI:10.1002/1096-9098(200010)75:2<98::aid-jso5>3.0.co;2-r

PMID:11064388

Abstract

BACKGROUND AND OBJECTIVES

To improve the survival rate of patients with colon cancer, liver metastases must be eradicated in a clinically occult state. This study was designed to find a predictor for potential liver metastases or micrometastases in colon cancer.

METHODS

Peripheral blood samples and tumor specimens were obtained from 36 patients with colon cancers. The blood samples were subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) analysis, and the expression of sialylated carbohydrates was also investigated in the tumors immunohistochemically.

RESULTS

A carcinoembryonic antigen (CEA)-specific signal in the blood was detected in 9 of 12 (75%) patients with liver metastasis and in 8 of 24 (33%) patients without liver metastasis, respectively (P < 0.05). The positive rates of sialyl Lewis A (sLeA) and sialyl Lewis X (sLeX) were 36.3% and 40% in tumors without liver metastasis vs. 58.3% and 100% with liver metastasis, respectively. Within a year after surgery, liver metastases became clinically evident in three of the four patients without liver metastasis who showed a CEA-positive signal in their blood preoperatively and who had tumors with a strong expression of sLeX.

CONCLUSIONS

A combination of both markers may provide prognostic information for liver metastases in colon cancer.

摘要

背景与目的

为提高结肠癌患者的生存率，必须在临床隐匿状态下根除肝转移灶。本研究旨在寻找结肠癌潜在肝转移或微转移的预测指标。

方法

从36例结肠癌患者中获取外周血样本和肿瘤标本。对血样进行逆转录聚合酶链反应（RT-PCR）分析，并通过免疫组织化学方法研究肿瘤中唾液酸化碳水化合物的表达。

结果

在12例有肝转移的患者中，有9例（75%）血中检测到癌胚抗原（CEA）特异性信号，而在24例无肝转移的患者中，有8例（33%）检测到该信号（P<0.05）。无肝转移肿瘤中唾液酸化路易斯A（sLeA）和唾液酸化路易斯X（sLeX）的阳性率分别为36.3%和40%，而有肝转移肿瘤中的阳性率分别为58.3%和100%。术后一年内，术前血中CEA信号阳性且肿瘤sLeX表达强的4例无肝转移患者中有3例出现了临床明显的肝转移。