Ward K M, Balaban R S
Laboratory of Cardiac Energetics, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
Magn Reson Med. 2000 Nov;44(5):799-802. doi: 10.1002/1522-2594(200011)44:5<799::aid-mrm18>3.0.co;2-s.
Solution pH was measured using water proton NMR via chemical exchange dependent saturation transfer (CEST) with selected chemical exchange sites. Several useful pH-sensitive proton chemical exchange agents were found: 5,6-dihydrouracil, 5-hydroxytryptophan, and a combination of 5-hydroxytryptophan and 2-imidazolidinethione. A ratiometric approach was developed that permitted pH determinations that were independent of water T(1) or exchange site concentration.
通过与选定化学交换位点的化学交换依赖饱和转移（CEST），使用水质子核磁共振测量溶液pH值。发现了几种有用的对pH敏感的质子化学交换剂：5,6 - 二氢尿嘧啶、5 - 羟色氨酸以及5 - 羟色氨酸和2 - 咪唑烷硫酮的组合。开发了一种比率法，可实现独立于水T(1)或交换位点浓度的pH测定。
