Dua V K, Gupta N C, Kar P K, Nand J, Edwards G, Sharma V P, Subbarao S K
Malaria Research Centre, Field Station, BHEL, Ranipur, Hardwar, India.
Ann Trop Med Parasitol. 2000 Sep;94(6):565-70. doi: 10.1080/00034983.2000.11813579.
The sensitivities of 61 Indian cases of Plasmodium falciparum malaria to chloroquine (CQ) were investigated using in-vitro and in-vivo methods. Concentrations of CQ and desethylchloroquine (DCQ) in blood cells and plasma from CQ-sensitive and -resistant cases were determined 2 and 7 days after initiation of treatment, by HPLC. On day 2, the mean CQ concentrations in the samples collected from the sensitive cases were higher than those in the samples from the resistant patients, in plasma (0.47 v. 0.32 microgram/ml; P < 0.02) and particularly in the blood cells (1.51 v. 0.46 micrograms/ml; P < 0.001). By day 7, however, the CQ concentrations in the two groups were similar. Although, on day 2, the mean ratio of the CQ to DCQ concentrations was significantly higher in the blood cells from the sensitive group than in those from the resistant cases (P < 0.01), the CQ/DCQ ratios for the plasma were similar for the two groups. Similarly, the mean ratio between the blood-cell concentration of CQ on day 2 and the concurrent plasma concentration (BPr) was also relatively high in the sensitive group (P < 0.001).
采用体外和体内方法对61例印度恶性疟原虫疟疾患者对氯喹（CQ）的敏感性进行了研究。通过高效液相色谱法（HPLC）测定了CQ敏感和耐药患者治疗开始后2天和7天血细胞和血浆中CQ和去乙基氯喹（DCQ）的浓度。第2天，从敏感患者采集的样本中血浆CQ平均浓度高于耐药患者样本（0.47对0.32微克/毫升；P<0.02），尤其是血细胞中（1.51对0.46微克/毫升；P<0.001）。然而，到第7天，两组的CQ浓度相似。虽然在第2天，敏感组血细胞中CQ与DCQ浓度的平均比值显著高于耐药患者（P<0.01），但两组血浆的CQ/DCQ比值相似。同样，敏感组第2天血细胞中CQ浓度与同期血浆浓度之比（BPr）也相对较高（P<0.001）。
