Karim E A, Ibrahim K E, Hassabalrasoul M A, Saeed B O, Bayoumi R A
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Khartoum, Sudan.
J Pharm Biomed Anal. 1992 Feb-Mar;10(2-3):219-23. doi: 10.1016/0731-7085(92)80032-i.
This study was carried out on 63 patients in the town of Gadaref in eastern Sudan; each patient was given the standard therapeutic dose of chloroquine (CQ). Plasma levels of chloroquine and its major metabolite desethylchloroquine (DCQ) were measured by means of a high-performance liquid chromatographic method (HPLC) in patients infected with sensitive (sensitive group) and resistant (resistant groups) strains of Plasmodium falciparum. The ratios of chloroquine to desethylchloroquine (CQ/DCQ) in different groups were calculated and the results obtained were compared and correlated with the degree of parasitaemia. The statistical analysis of the results showed that the plasma content of CQ and the CQ/DCQ ratio in the majority of the patients fall within the normal mode of distribution. A small group of patients showed a deviation from the normal mode by having a rather high CQ plasma level and a high ratio of CQ/DCQ. The mean plasma levels of CQ and the CQ/DCQ ratio in the sensitive group was found to be higher than that in the resistant groups. However, these differences were found to be not significant. Correlation tests showed that the levels of CQ and the CQ/DCQ ratios increase with the increase of the degree of parasitaemia in the sensitive group but decrease with the increase of parasitaemia in resistant groups.
本研究在苏丹东部加达里夫镇的63名患者中进行；每位患者均接受了标准治疗剂量的氯喹（CQ）。采用高效液相色谱法（HPLC）测定了感染恶性疟原虫敏感株（敏感组）和耐药株（耐药组）患者血浆中氯喹及其主要代谢产物去乙基氯喹（DCQ）的水平。计算了不同组中氯喹与去乙基氯喹的比值（CQ/DCQ），并将所得结果进行比较，并与疟原虫血症程度相关联。结果的统计分析表明，大多数患者血浆中CQ的含量以及CQ/DCQ比值处于正常分布模式。一小部分患者表现出与正常模式的偏差，其血浆中CQ水平相当高，且CQ/DCQ比值也很高。发现敏感组中CQ的平均血浆水平和CQ/DCQ比值高于耐药组。然而，这些差异并不显著。相关性测试表明，敏感组中CQ水平和CQ/DCQ比值随疟原虫血症程度的增加而升高，但在耐药组中随疟原虫血症的增加而降低。
