Chang C W, Wang H E, Lin H M, Chtsai C S, Chen J B, Liu R S
National PET/Cyclotron Center, Taipei Veterans General Hospital, and National Yang-Ming University School of Medicine, Taiwan.
Nucl Med Commun. 2000 Sep;21(9):799-802. doi: 10.1097/00006231-200009000-00003.
The aim of this study was to develop a method of semi-automated 6-[18F]fluoro-L-dopa (6-[18F]FDOPA) synthesis using a robotic system (Scanditronix Anatech RB III, Uppsala, Sweden). [18F]Fluorine was produced via 20Ne(d,alpha)18F using a Scanditronix MC17F cyclotron (Uppsala). The radiosynthesis was performed by the Scanditronix Anatech RB III robotic system. On average, a typical run produced 16-19 mCi of 6-[18F]FDOPA at end of synthesis (EOS) after 2 h irradiation of the F2/neon gas target. The total synthesis time was 110 min. The retention time of 6-[18F]FDOPA (the radio peak) was 8.2 min, which was consistent with the 6-[19F]FDOPA ultraviolet peak. The radiochemical purity was greater than 97%. A robotic, semi-automated method for 6-[18F]FDOPA radiosynthesis is therefore feasible. The radiation burden for the operator can be reduced as much as possible. Sufficient activity of 6-[18F]FDOPA could be obtained for positron emission tomography studies of dopaminergic function.
本研究的目的是开发一种使用机器人系统（瑞典乌普萨拉的Scanditronix Anatech RB III）半自动合成6-[18F]氟-L-多巴（6-[18F]FDOPA）的方法。[18F]氟通过使用Scanditronix MC17F回旋加速器（乌普萨拉）的20Ne（d，α）18F反应产生。放射性合成由Scanditronix Anatech RB III机器人系统进行。在对F2/氖气靶进行2小时辐照后，典型的一次运行在合成结束时（EOS）平均产生16 - 19毫居里的6-[18F]FDOPA。总合成时间为110分钟。6-[18F]FDOPA的保留时间（放射性峰）为8.2分钟，这与6-[19F]FDOPA的紫外峰一致。放射化学纯度大于97%。因此，一种用于6-[18F]FDOPA放射性合成的机器人半自动方法是可行的。可以尽可能降低操作人员的辐射负担。可以获得足够活性的6-[18F]FDOPA用于多巴胺能功能的正电子发射断层扫描研究。
