Hofbauer R, Frass M, Gmeiner B, Sandor N, Schumann R, Wagner O, Kaye A D
Department of Internal Medicine, MICU, University of Vienna, Austria.
Acta Anaesthesiol Scand. 2000 Nov;44(10):1232-7. doi: 10.1034/j.1399-6576.2000.441008.x.
Anaesthetic drugs are used for pain therapy and anaesthesia. Neutrophils play a significant role during the process of inflammation. The aim of the current study was to investigate the effects of remifentanil and fentanyl on neutrophil migration through endothelial cell monolayers, and on adhesion molecule expression.
After isolation of polymorphonuclear neutrophils (PMNL) we used a currently described migration assay. PMNL and/or endothelial cell monolayers (ECM) were pre-treated with remifentanil using clinically relevant, as well as higher and lower concentrations or relevant concentrations of fentanyl.
Concentrations of remifentanil (50 ng/mL) similar to the relevant plasma concentration were able to inhibit PMNL migration through ECM significantly (migration compared to the control 82+/-7% SD; P<0.05), when both cell types were treated with the synthetic narcotic remifentanil. Fentanyl (30 ng/mL) showed a stronger inhibitory effect (migration compared to the control 67+/-9.2%; P<0.05). Endothelial cell adhesion molecule expression was reduced after either remifentanil or fentanyl.
The results of the present investigation indicate that remifentanil influences interaction of ECM against human neutrophils. Compared to fentanyl, remifentanil seems to exhibit minor inhibitory effects on neutrophil migration.
麻醉药物用于疼痛治疗和麻醉。中性粒细胞在炎症过程中起重要作用。本研究的目的是探讨瑞芬太尼和芬太尼对中性粒细胞通过内皮细胞单层迁移以及对黏附分子表达的影响。
分离多形核中性粒细胞（PMNL）后，我们使用了目前描述的迁移试验。用临床相关浓度、更高和更低浓度的瑞芬太尼或相关浓度的芬太尼对PMNL和/或内皮细胞单层（ECM）进行预处理。
当两种细胞类型都用合成麻醉剂瑞芬太尼处理时，与相关血浆浓度相似的瑞芬太尼浓度（50 ng/mL）能够显著抑制PMNL通过ECM的迁移（与对照组相比迁移率为82±7%标准差；P<0.05）。芬太尼（30 ng/mL）显示出更强的抑制作用（与对照组相比迁移率为67±9.2%；P<0.05）。瑞芬太尼或芬太尼处理后内皮细胞黏附分子表达降低。
本研究结果表明瑞芬太尼影响ECM与人类中性粒细胞的相互作用。与芬太尼相比，瑞芬太尼似乎对中性粒细胞迁移表现出较小的抑制作用。
