Hofbauer Roland, Frass Michael, Pasching Eva, Gmeiner Bernhard, Kaye Alan D, Kapiotis Stylianos
Clinical Institute of Medical and Chemical Laboratory Diagnostics, University Hospital, University of Vienna, Austria.
J Toxicol Environ Health A. 2002 May 10;65(9):685-93. doi: 10.1080/15287390252900386.
Furosemide and spironolactone reduce transmigration of leukocytes through endothelial cell monolayers. Leukocytes play a tremendous role during inflammation. Leukocytes migrate from intravascular space into the tissue to attack microorganisms. Various agents are able to influence leukocyte recruitment. The influence of diuretics, such as furosemide and spironolactone, on inflammatory processes is not well known. The aim of our study was to examine the influence of furosemide and spironolactone on leukocyte migration through endothelial cell monolayers (ECM). Human umbilical vein endothelial cells were cultured on microporous membranes achieving a monolayer. Polymorphonuclear leukocytes (PMNL) were used in a currently described migration assay. PMNL and/or ECM were pretreated with furosemide and spironolactone using therapeutic, as well as higher and lower, concentrations. Furosemide (76 +/- 7.2%) and spironolactone (70 +/- 7.7%) were able to inhibit PMNL migration through ECM significantly, when both cell types were treated simulating the situation after an iv injection. Furosemide and spironolactone were identified as potent inhibitors of leukocyte migration through ECM.
呋塞米和螺内酯可减少白细胞通过内皮细胞单层的迁移。白细胞在炎症过程中发挥着巨大作用。白细胞从血管内空间迁移到组织中以攻击微生物。多种因素能够影响白细胞募集。利尿剂如呋塞米和螺内酯对炎症过程的影响尚不清楚。我们研究的目的是检测呋塞米和螺内酯对白细胞通过内皮细胞单层（ECM）迁移的影响。人脐静脉内皮细胞在微孔膜上培养形成单层。在当前描述的迁移试验中使用多形核白细胞（PMNL）。使用治疗浓度以及更高和更低浓度的呋塞米和螺内酯对PMNL和/或ECM进行预处理。当两种细胞类型都进行处理以模拟静脉注射后的情况时，呋塞米（76±7.2%）和螺内酯（70±7.7%）能够显著抑制PMNL通过ECM的迁移。呋塞米和螺内酯被确定为白细胞通过ECM迁移的有效抑制剂。
