Lopes M C, Ramoni C
Boll Ist Sieroter Milan. 1975 Jun 26;54(2):82-9.
The multiplication of Bhanja virus in CV-1 cells was studied both by staining the viral antigens with the FA technique and measuring the light intensity emitted by the fluorescent cells with a photomultiplier and by the simultaneous titration of intracellular and extracellular virus. The fluorescence appeared at 3 hrs post adsorption in the form of very small granules in the cytoplasm of the infected cells. Percentage of cells containing viral antigens reached its maximum at 18 hrs post adsorption. Later on the fluorescence slowly decreased. The virus in infected cells was demonstrated 9 hrs post adsorption and maximum titre was reached 48 hrs post adsorption. The cell sheet remained apparently normal and no sign of cytopathic effect was observed until 36 hrs post adsorption.
通过用荧光抗体（FA）技术对病毒抗原进行染色并用光电倍增管测量荧光细胞发出的光强度，以及同时对细胞内和细胞外病毒进行滴定，研究了班贾病毒在CV-1细胞中的增殖情况。吸附后3小时，荧光以非常小的颗粒形式出现在受感染细胞的细胞质中。含有病毒抗原的细胞百分比在吸附后18小时达到最大值。随后荧光缓慢下降。吸附后9小时在受感染细胞中检测到病毒，吸附后48小时达到最高滴度。直到吸附后36小时，细胞单层仍明显正常，未观察到细胞病变效应的迹象。
