Dea S, Elazhary M A
Cornell Vet. 1984 Jul;74(3):208-17.
The porcine adenovirus type 4 was adapted to grow in porcine thyroid cell cultures. A readily recognizable cytopathic effect appeared in these cells as soon as the first passage of the virus and complete degeneration of the monolayers was obtained after only 72 hours post-infection at the fourth passage. A viral yield of 10(6.0) TCID50/ml was calculated after the third passage. The virus was purified by CsCl density gradient centrifugation and was shown to possess a buoyant density of 1.33 g/ml. A specific antiserum was prepared from two specific-pathogen-free piglets and used for indirect immunofluorescent staining. The fluorescence was observed in the nucleus of infected cells at 24 to 72 hours post-inoculation. The use of TP cells is suggested for routine porcine adenovirus diagnosis.
猪4型腺病毒经适应后可在猪甲状腺细胞培养物中生长。在病毒首次传代后，这些细胞中很快就出现了易于识别的细胞病变效应，在第四次传代感染后仅72小时，单层细胞就完全退化。第三次传代后计算出病毒产量为10(6.0) TCID50/ml。该病毒通过氯化铯密度梯度离心法纯化，其浮力密度为1.33 g/ml。从两只无特定病原体的仔猪制备了特异性抗血清，并用于间接免疫荧光染色。接种后24至72小时，在感染细胞的细胞核中观察到荧光。建议使用TP细胞进行常规猪腺病毒诊断。
