Bonaduce D, Martone F, De Caprio L
Boll Ist Sieroter Milan. 1975 Oct 20;54(4):253-71.
The Authors have researched to adapt Orthomyxovirus, subtype A/equi-2 (Naples/213/67, Andria/60/68 and Miami/63), by serial passages on kidney calf cells, chick embryo cells and RK-13 cell line or by one or two passages on these colture media alternated with one passage "in ovo". The results obtained show that only kidney calf cells are most suitable because the viruses replicate for eleven passages. On the chick embryo cells and RK-13 cell line the viruses replicate only for two passages and these results are not modified when one or two passages are alternated with one passage "in ovo". The Authors have observed the production of infectious but not haemadsorbing and haemagglutining virus. The phenomenon is discussed and integrated with collateral tests.
作者们通过在小牛肾细胞、鸡胚细胞和RK - 13细胞系上连续传代，或在这些培养基上传代一两次后再进行一次“卵内”传代，对A型正粘病毒（A/equi - 2亚型，那不勒斯/213/67、安德里亚/60/68和迈阿密/63）进行适应性研究。所获得的结果表明，只有小牛肾细胞最为合适，因为病毒能在其上复制11代。在鸡胚细胞和RK - 13细胞系上，病毒仅能复制两代，而且当一两次传代与一次“卵内”传代交替进行时，这些结果并未改变。作者们观察到了有感染性但无血细胞吸附和血凝性病毒的产生。对这一现象进行了讨论，并结合相关试验进行了综合分析。
