使用辣根过氧化物酶标记抗血清在实验性慢性血清病中证明肾小球基底膜中存在含免疫球蛋白的沉积物。
Demonstration of immunoglobulin containing deposits in glomerular basement membrane in experimental chronic serum sickness using horseradish peroxidase labelled antiserum.
作者信息
Davies D R, Clark A E
出版信息
Br J Exp Pathol. 1975 Feb;56(1):28-33.
Abstract
Horseradish peroxidase (HRP) labelled antibodies were used in rabbit glomerulonephritis to obtain localization of immunoglobulins in the glomerulus at a light and electron microscope level. Better localization of deposits was obtained with peroxidase labelled antiserum than with immunofluorescence at the light microscope level. Ultrastructurally, immunoglobulin localization was seen to correspond to the electron dense deposits demonstrated in and on the glomerular basement membrane by conventional microscopy.
摘要
辣根过氧化物酶（HRP）标记的抗体被用于兔肾小球肾炎研究，以在光学显微镜和电子显微镜水平上获得免疫球蛋白在肾小球中的定位。在光学显微镜水平上，与免疫荧光法相比，过氧化物酶标记的抗血清能更有效地定位沉积物。在超微结构上，免疫球蛋白的定位与传统显微镜观察到的肾小球基底膜内和膜上的电子致密沉积物一致。
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