Sonea I M, Jergens A E, Sacco R E, Niyo Y, Merten E, Kauffman L K, Moore P F
Department Biomedical Sciences, College of Veterinary Medicine, 2008 Veterinary Medicine Building, Iowa State University, Ames, IA 50011, USA.
Vet Immunol Immunopathol. 2000 Nov 23;77(1-2):103-19. doi: 10.1016/s0165-2427(00)00230-0.
Flow cytometric analysis of the lymphocyte population of the gut could provide useful information on the immune cells present in the gut that would not be easily obtained in tissue sections. However, little is known of the normal lymphocyte population in the canine gut as determined by flow cytometry, which allows for simultaneous staining of multiple cell surface antigens and identification of specific lymphocytic subsets. Therefore, intraepithelial lymphocytes were obtained from biopsies of the healthy canine proximal small intestine and colon taken with an endoscope, and flow cytometric analysis was used to characterize the lymphocyte subsets present. Endoscopic biopsy of the intestine is a minimally invasive technique commonly used for diagnostic purposes. Although CD3+ lymphocytes were the most abundant subset in both colon and small intestine, CD3+/CD8- lymphocytes predominated in the proximal small intestine, whereas CD3+/CD8+ lymphocytes did in the colon. Canine CD8+ intraepithelial lymphocytes were predominantly CD8alphabeta+ in both small intestine and colon. CD4+ intraepithelial lymphocytes were always much less numerous than CD8+ intraepithelial lymphocytes. As in man, a majority of intraepithelial lymphocytes expressed the T-cell receptor, TCRalphabeta, but TCRgammadelta was expressed by a third of intraepithelial T-cells in the proximal small intestine, and approximately 15% of those in the colon. Very few CD21+ lymphocytes were detected in samples of healthy canine colon and small intestinal intraepithelial cells. We have showed that canine intraepithelial lymphocytes are regionally specialized, and that those from the small intestine are unique in comparison to those of other species such as man and rodents due to the large numbers of CD3+/CD8- intraepithelial lymphocytes. This study provides a baseline for comparison with intraepithelial lymphocytes obtained from canine patients with intestinal disease.
对肠道淋巴细胞群体进行流式细胞术分析，可以提供有关肠道中存在的免疫细胞的有用信息，而这些信息在组织切片中不易获得。然而，通过流式细胞术确定的犬肠道正常淋巴细胞群体情况却鲜为人知，流式细胞术能够同时对多种细胞表面抗原进行染色，并识别特定的淋巴细胞亚群。因此，从通过内窥镜获取的健康犬近端小肠和结肠活检组织中获取上皮内淋巴细胞，并采用流式细胞术分析来表征存在的淋巴细胞亚群。肠道内窥镜活检是一种常用于诊断目的的微创技术。虽然CD3 +淋巴细胞是结肠和小肠中最丰富的亚群，但CD3 + / CD8 -淋巴细胞在近端小肠中占主导地位，而CD3 + / CD8 +淋巴细胞在结肠中占主导地位。犬CD8 +上皮内淋巴细胞在小肠和结肠中主要为CD8αβ +。CD4 +上皮内淋巴细胞的数量总是比CD8 +上皮内淋巴细胞少得多。与人类一样，大多数上皮内淋巴细胞表达T细胞受体TCRαβ，但三分之一的近端小肠上皮内T细胞和大约15%的结肠上皮内T细胞表达TCRγδ。在健康犬结肠和小肠上皮内细胞样本中检测到的CD21 +淋巴细胞极少。我们已经表明，犬上皮内淋巴细胞具有区域特异性，并且由于大量的CD3 + / CD8 -上皮内淋巴细胞，与人类和啮齿动物等其他物种相比，来自小肠的上皮内淋巴细胞是独特的。这项研究为与从患有肠道疾病的犬患者获得的上皮内淋巴细胞进行比较提供了一个基线。
