Mosley R L, Styre D, Klein J R
Department of Biological Science, University of Tulsa, OK 74104.
Int Immunol. 1990;2(4):361-5. doi: 10.1093/intimm/2.4.361.
We have studied a population of CD4+ intestinal intraepithelial lymphocytes using two-color flow cytometric analyses, and in highly purified fluorescent-activated cell-sorted preparations. Although CD4+ T cells present within the epithelial immune compartment comprised only approximately 10-20% of the total intestinal epithelial lymphoid cells, an unusually high proportion of those CD4+ lymphocytes expressed a CD4+CD8+ phenotype which is rarely encountered in peripheral T cells. By comparison, CD4+ lymphocytes from spleen or lymph nodes existed exclusively as single-positive T cells. Analyses of CD4 and CD8 expression on lymphocytes from Peyer's patches, the lamina propria, and IEL indicated that CD4+CD8+ lymphocytes were unique to the IEL. Using CD4+CD8+ preparations obtained by fluorescent-activated cell sorting, CD4+CD8+ epithelial T cells were found also to express CD3 and Thy-1 surface markers. This heretofore undescribed extrathymic population of double-positive T cells constitutes a unique peripheral T cell subset which may be involved in intestinal T cell maturation and development, or could represent a highly specialized effector population.
我们使用双色流式细胞术分析，并在高度纯化的荧光激活细胞分选制剂中，研究了一群CD4⁺肠上皮内淋巴细胞。尽管存在于上皮免疫区室中的CD4⁺T细胞仅占肠上皮淋巴样细胞总数的约10%-20%，但这些CD4⁺淋巴细胞中异常高比例表达CD4⁺CD8⁺表型，而这种表型在外周T细胞中很少见。相比之下，来自脾脏或淋巴结的CD4⁺淋巴细胞仅作为单阳性T细胞存在。对派尔集合淋巴结、固有层和肠上皮内淋巴细胞中淋巴细胞的CD4和CD8表达分析表明，CD4⁺CD8⁺淋巴细胞是肠上皮内淋巴细胞所特有的。使用通过荧光激活细胞分选获得的CD4⁺CD8⁺制剂，发现CD4⁺CD8⁺上皮T细胞也表达CD3和Thy-1表面标志物。这种迄今为止未描述的双阳性T细胞的胸腺外群体构成了一个独特的外周T细胞亚群，其可能参与肠道T细胞的成熟和发育，或者可能代表一个高度特化的效应细胞群体。
