Waner T, Leykin I, Shinitsky M, Sharabani E, Buch H, Keysary A, Bark H, Harrus S
Israel Institute for Biological Research, PO Box 19, Ness Ziona, Israel.
Vet Immunol Immunopathol. 2000 Nov 23;77(1-2):145-50. doi: 10.1016/s0165-2427(00)00225-7.
Six dogs were infected with Ehrlichia canis by intravenous injection of heavily infected DH82 cells. All dogs developed typical signs of canine monocytic ehrlichiosis. Using flow cytometric technology, platelet-bound IgG (PBIgG) were detected in 5 of the 6 dogs after experimental infection with E. canis over a period of 3-10 days post infection (PI). The first detection of PBIgG was made as early as day 3 PI in 2 out of 6 dogs, and on day 5 PI in 1 dog. On day 7 PI, PBIgG was detected in 2 dogs, and on day 10 PI in 3 out of 6 dogs. This is the first report documenting the presence of PBIgG following E. canis infection in dogs. This finding further supports the theory that the thrombocytopenia seen in canine monocytic ehrlichiosis has an immunological component and that exposure to an infectious agent, in this case the rickettsia E. canis, can trigger autoimmune mechanisms. Due to the heterogeneous appearance of PBIgG among the infected dogs it was concluded that other non-immunological mechanisms are probably also involved in the pathogenesis of the thrombocytopenia seen in canine monocytic ehrlichiosis.
通过静脉注射大量感染的DH82细胞，使6只狗感染犬埃立克体。所有狗都出现了犬单核细胞埃立克体病的典型症状。利用流式细胞术，在6只狗中的5只狗在感染犬埃立克体后的3至10天内检测到血小板结合免疫球蛋白（PBIgG）。最早在感染后第3天在6只狗中的2只狗中检测到PBIgG，在第5天在1只狗中检测到。在感染后第7天，在2只狗中检测到PBIgG，在第10天在6只狗中的3只狗中检测到。这是第一份记录犬感染犬埃立克体后PBIgG存在情况的报告。这一发现进一步支持了这样一种理论，即犬单核细胞埃立克体病中出现的血小板减少症具有免疫成分，并且接触感染因子，在这种情况下是立克次氏体犬埃立克体，可触发自身免疫机制。由于感染狗中PBIgG的表现存在异质性，得出的结论是，其他非免疫机制可能也参与了犬单核细胞埃立克体病中血小板减少症的发病机制。
