Kwon H M, Hong B K, Kang T S, Kwon K, Kim H K, Jang Y, Choi D, Park H Y, Kang S M, Cho S Y, Kim H S
Yonsei Cardiovascular Center and Research Institute, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
J Korean Med Sci. 2000 Oct;15(5):485-93. doi: 10.3346/jkms.2000.15.5.485.
Advanced atherosclerosis is often associated with dystrophic calcification and remodeling of extracellular matrix of vascular wall. Recently many studies have documented a general relationship between calcification and severity of coronary disease, and discussed the feasibility of electron beam computed tomography for detecting and quantifying the coronary artery calcification in the patients. The present study investigated the expression and the localization of osteopontin, one of noncollagenous bone matrix protein, within the calcified coronary arteries. Autopsy-derived coronary artery specimens were scanned and reconstructed to visualize the pattern of coronary calcification using a novel microscopic computed tomography technique. The localization of the osteopontin were evaluated by immunohistochemial stain with LF7. The present study showed that the pattern of coronary calcification is variable and the expression of osteopontin is localized mainly to calcified lesion. The smooth muscle cells in addition to macrophage expressed osteopontin protein in human coronary atherosclerotic plaques. Soluble osteopontin released near to the sites of vascular calcification may represent an adaptive mechanism aimed at regulating the process of vascular calcification.
晚期动脉粥样硬化常与血管壁细胞外基质的营养不良性钙化和重塑相关。最近，许多研究记录了钙化与冠状动脉疾病严重程度之间的普遍关系，并讨论了电子束计算机断层扫描在检测和量化患者冠状动脉钙化方面的可行性。本研究调查了非胶原骨基质蛋白之一骨桥蛋白在钙化冠状动脉内的表达和定位。使用一种新型显微计算机断层扫描技术对尸检获得的冠状动脉标本进行扫描和重建，以可视化冠状动脉钙化模式。通过用LF7进行免疫组织化学染色来评估骨桥蛋白 的定位。本研究表明，冠状动脉钙化模式是可变的，骨桥蛋白的表达主要定位于钙化病变处。除巨噬细胞外，平滑肌细胞在人类冠状动脉粥样硬化斑块中也表达骨桥蛋白。在血管钙化部位附近释放的可溶性骨桥蛋白可能代表一种旨在调节血管钙化过程的适应性机制。
