Gerendai I, Tóth I E, Boldogkoi Z, Medveczky I, Halász B
Hungarian Academy of Sciences and Semmelweis University, Department of Human Morphology and Developmental Biology, Budapest.
J Neuroendocrinol. 2000 Nov;12(11):1087-95. doi: 10.1046/j.1365-2826.2000.00560.x.
In the present study, the transneuronal transport of neurotrophic virus technique was used to identify cell groups of the spinal cord and the brain that are transsynaptically connected with the testis. Pseudorabies virus was injected into the testis and after survival times of 3-6 days, the spinal cord and brain were processed immunocytochemically using a polyclonal antibody against the virus. Virus-infected perikarya were detected in the preganglionic neurones of the spinal cord (T10-L1, L5-S1) and in certain cell groups and areas of the brain stem, the hypothalamus and the telencephalon. In the brain stem, the cell groups and areas in which labelled neurones were present included, among others, the nucleus of the solitary tract, the caudal raphe nuclei, the locus coeruleus and the periaqueductal grey of the mesencephalon. In the hypothalamus, virus infected perikarya were observed in the paraventricular nucleus and in certain other cell groups. Telencephalic structures containing labelled neurones included the preoptic area, the bed nucleus of the stria terminalis, the central amygdala and the insular cortex. These data identify a multisynaptic circuit of neurones in the spinal cord and in the brain which may be involved in the control of testicular functions.
在本研究中，运用神经营养病毒的跨神经元运输技术来鉴定脊髓和大脑中与睾丸存在跨突触连接的细胞群。将伪狂犬病病毒注入睾丸，在3 - 6天的存活期后，使用针对该病毒的多克隆抗体对脊髓和大脑进行免疫细胞化学处理。在脊髓的节前神经元（胸10 - 腰1、腰5 - 骶1）以及脑干、下丘脑和端脑的某些细胞群和区域中检测到了病毒感染的神经细胞体。在脑干中，存在标记神经元的细胞群和区域包括孤束核、尾侧中缝核、蓝斑以及中脑导水管周围灰质等。在下丘脑中，在室旁核和其他某些细胞群中观察到了病毒感染的神经细胞体。含有标记神经元的端脑结构包括视前区、终纹床核、中央杏仁核和岛叶皮质。这些数据确定了脊髓和大脑中可能参与睾丸功能控制的多突触神经元回路。
