Weil G J, Steel C, Liftis F, Li B W, Mearns G, Lobos E, Nutman T B
Infectious Diseases Division, Barnes-Jewish Hospital, St. Louis, MO 63110, USA.
J Infect Dis. 2000 Dec;182(6):1796-9. doi: 10.1086/317629. Epub 2000 Oct 26.
Improved methods are needed for field diagnosis of onchocerciasis, to support efforts aimed at elimination of the disease. A rapid-format card test was evaluated that detects IgG4 antibodies to recombinant Onchocerca volvulus antigen Ov16 with serum samples from patients with onchocerciasis and with various types of control serum samples. The sensitivity of the test with serum samples from 106 microfilariae-positive subjects was 90.6%. The test was equally sensitive with serum samples obtained from patients in Africa and Latin America. Specificity was excellent; positive tests were observed for 2 of 38 serum samples from patients with other filarial infections and for 1 of 23 serum samples from patients with nonfilarial helminth infections. The 3 "false-positive" serum samples were from West Africans who could have been coinfected with onchocerciasis. No positive tests were observed with nonendemic serum samples from normal adults, patients with autoimmune disorders, or patients with the hyper-IgE syndrome. This new test holds great promise as a simple tool for diagnosis of onchocerciasis.
需要改进盘尾丝虫病的现场诊断方法，以支持旨在消除该疾病的努力。对一种快速检测卡进行了评估，该检测卡利用盘尾丝虫病患者的血清样本以及各种类型的对照血清样本，检测针对重组盘尾丝虫抗原Ov16的IgG4抗体。对106名微丝蚴阳性受试者的血清样本进行检测，该检测的灵敏度为90.6%。对从非洲和拉丁美洲患者获得的血清样本，该检测同样灵敏。特异性极佳；在38份来自其他丝虫感染患者的血清样本中有2份检测呈阳性，在23份来自非丝虫性蠕虫感染患者的血清样本中有1份检测呈阳性。这3份“假阳性”血清样本来自可能同时感染盘尾丝虫病的西非患者。正常成年人、自身免疫性疾病患者或高IgE综合征患者的非流行地区血清样本检测均未呈阳性。作为一种诊断盘尾丝虫病的简单工具，这项新检测极具前景。
