Fitzgerald M P, Mollenhauer J, Brubaker L
Department of Obstetrics/Gynecology, Division of Urogynecology and Reconstructive Pelvic Surgery, Rush-Presbyterian-St-Luke's Medical Center, Chicago, IL 60612-3865, USA.
BJU Int. 2000 Nov;86(7):826-8. doi: 10.1046/j.1464-410x.2000.00917.x.
To determine whether commercially available fascia lata allograft material contains donor antigens.
Human leukocyte antigens (HLA) class I and II were assessed in: (i) freeze-dried fascia lata allografts; (ii) a Tutoplast fascia lata graft (Mentor Urology, Santa Barbara, CA, USA); (iii) an acellular dermal graft; and (iv) a successful donor fascia sacrocolpopexy graft one year after implantation, using a polymerase chain reaction sequence-specific primer-based assay.
The donor for both the freeze-dried fascia lata and Tutoplast fascia lata was fully HLA-typed. At one year after implantation, antigens from the implanted sacrocolpopexy graft matched the host blood antigens. The antigenicity of the acellular dermal graft could not be ascertained because this material interfered with the assay.
Donor fascia lata grafts prepared by freeze-drying or by the Tutoplast technique retain donor antigens. The significance of this antigenicity is unknown. All donor antigens are replaced by host antigens after implantation.
确定市售阔筋膜同种异体移植材料是否含有供体抗原。
采用基于聚合酶链反应序列特异性引物的检测方法，对以下材料进行人类白细胞抗原（HLA）Ⅰ类和Ⅱ类评估：（i）冻干阔筋膜同种异体移植物；（ii）Tutoplast阔筋膜移植物（美国加利福尼亚州圣巴巴拉市Mentor泌尿外科公司）；（iii）脱细胞真皮移植物；（iv）植入一年后的成功供体阔筋膜骶骨阴道固定术移植物。
冻干阔筋膜和Tutoplast阔筋膜的供体均进行了完整的HLA分型。植入后一年，植入的骶骨阴道固定术移植物的抗原与宿主血液抗原相匹配。由于该材料干扰检测，脱细胞真皮移植物的抗原性无法确定。
通过冻干或Tutoplast技术制备的供体阔筋膜移植物保留了供体抗原。这种抗原性的意义尚不清楚。植入后所有供体抗原均被宿主抗原取代。
