Lee C W
Department of Dermatology, Hanyang University Hospital, Sungdong-ku, Seoul 133-792, Korea.
Br J Dermatol. 2000 Oct;143(4):821-3. doi: 10.1046/j.1365-2133.2000.03838.x.
Autoantibodies that are characteristic of autoimmune bullous diseases (AIBDs) can be detected by immunoblot assay using epidermal or dermal extracts. However, none of the substrates obtained by ordinary methods seems to contain a sufficient amount of all of the autoantigens involved in AIBDs, and diagnosis may require the use of several different substrates.
To examine the potential of A431 cell extracts as a substrate for immunoblotting.
Fourteen sera obtained from patients with major AIBDs (pemphigus vulgaris, pemphigus foliaceus, paraneoplastic pemphigus, bullous pemphigoid and epidermolysis bullosa acquisita) were tested using this substrate.
Bands corresponding to desmoglein 1 and 3, desmoplakin 1 and 2, periplakin, BPAG1, BPAG2 and type VII collagen were identified distinctly with these sera.
This finding suggests that culture extracts of A431 cells provide an effective substrate for the diagnosis and differential diagnosis of major AIBDs.
自身免疫性大疱性疾病（AIBDs）的特征性自身抗体可通过使用表皮或真皮提取物的免疫印迹法检测。然而，通过常规方法获得的底物似乎都不含有足够量的所有与AIBDs相关的自身抗原，并且诊断可能需要使用几种不同的底物。
研究A431细胞提取物作为免疫印迹底物的潜力。
使用该底物检测了从主要AIBDs患者（寻常型天疱疮、落叶型天疱疮、副肿瘤性天疱疮、大疱性类天疱疮和获得性大疱性表皮松解症）获得的14份血清。
用这些血清可清晰地鉴定出与桥粒芯糖蛋白1和3、桥粒斑蛋白1和2、周斑蛋白、BPAG1、BPAG2和VII型胶原相对应的条带。
这一发现表明A431细胞的培养提取物为主要AIBDs的诊断和鉴别诊断提供了一种有效的底物。
