Solfert R, von der Haar F, Sternbach H, Sprinzl M, Cramer F
Hoppe Seylers Z Physiol Chem. 1975 Nov;356(11):1811-9.
The nucleobases adenine and cytosine were subjected to N-oxidation by monoperphthalic acid at pH 7 and 0 degrees C in tRNAPhe, in the 3'-terminal pentanucleotide C-A-C-C-A from tRNAPhe, as well as in tRNAVal and in the 3'-terminal nonanucleotide A-A-A-U-C-A-C-C-A from tRNAVal. In the tRNAs, the oxidative attack occurs stepwise from the 3'-end. First the 3'-terminal adenine is oxidized, then the following two cytosines, then the following adenine. The oligonucleotides are far more reactive than the identical sequences in tRNA and are not oxidized according to a sequential mechanism.
在pH值为7、0℃的条件下，单过氧邻苯二甲酸使苯丙氨酸转运核糖核酸（tRNAPhe）中的腺嘌呤和胞嘧啶发生N-氧化反应，该反应也发生在tRNAPhe的3'-末端五核苷酸C-A-C-C-A、缬氨酸转运核糖核酸（tRNAVal）以及tRNAVal的3'-末端九核苷酸A-A-A-U-C-A-C-C-A中。在转运核糖核酸中，氧化攻击从3'-末端逐步发生。首先是3'-末端的腺嘌呤被氧化，接着是随后的两个胞嘧啶，然后是再接下来的腺嘌呤。这些寡核苷酸比转运核糖核酸中相同序列的反应活性高得多，并且不是按照顺序机制被氧化的。
