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面包酵母tRNAVal 2b的一级结构。

Primary structure of baker's yeast tRNAVal 2b.

作者信息

Gorbulev V G, Axel'rod V D, Bayev A A

出版信息

Nucleic Acids Res. 1977 Sep;4(9):3239-58. doi: 10.1093/nar/4.9.3239.

DOI:10.1093/nar/4.9.3239
PMID:410008
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342647/
Abstract

The minor form of valine tRNA from baker's yeast-tRNAVal 2b--purified by column chromatography was completely digested with guanylo-RNase and pancreatic RNase. The products of these digestions were separated by a combination of thin-layer chromatography on cellulose and high voltage electrophoresis on DEAE-paper and then identified. The halves of tRNA Val 2b were prepared by partial digestion with pancreatic RNase, and their complete guanylo-RNase and pancreatic RNase digests were analysed. Basing on the obtained data the primary structure of baker's yeast tRNA Val 2b was reconstructed.

摘要

通过柱色谱法纯化的面包酵母缬氨酸tRNA的小形式——tRNAVal 2b，用鸟苷酸核糖核酸酶和胰核糖核酸酶完全消化。这些消化产物通过纤维素薄层层析和DEAE纸高压电泳相结合的方法进行分离，然后进行鉴定。用胰核糖核酸酶部分消化制备tRNA Val 2b的两半，并分析它们完整的鸟苷酸核糖核酸酶和胰核糖核酸酶消化产物。根据获得的数据重建了面包酵母tRNA Val 2b的一级结构。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/834a/342647/e96b13bc98da/nar00482-0301-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/834a/342647/8e5acc7b7e3a/nar00482-0295-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/834a/342647/9d89f25d436d/nar00482-0297-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/834a/342647/51718da871b4/nar00482-0300-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/834a/342647/e96b13bc98da/nar00482-0301-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/834a/342647/8e5acc7b7e3a/nar00482-0295-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/834a/342647/9d89f25d436d/nar00482-0297-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/834a/342647/51718da871b4/nar00482-0300-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/834a/342647/e96b13bc98da/nar00482-0301-a.jpg

相似文献

1
Primary structure of baker's yeast tRNAVal 2b.面包酵母tRNAVal 2b的一级结构。
Nucleic Acids Res. 1977 Sep;4(9):3239-58. doi: 10.1093/nar/4.9.3239.
2
The nucleotide sequence of cysteine transfer ribonucleic acid from baker's yeast. Products of complete digestion with pancreatic ribonuclease and ribonuclease T1.面包酵母半胱氨酸转移核糖核酸的核苷酸序列。用胰核糖核酸酶和核糖核酸酶T1完全消化的产物。
Biochem J. 1976 Feb 1;153(2):437-46. doi: 10.1042/bj1530437.
3
Nucleotide sequence in tRNA Val-2a from baker's yeast.来自面包酵母的缬氨酸tRNA Val-2a中的核苷酸序列。
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The primary structure of tRNAIIArg from brewers' yeast. 1. Complete digestions with pancreatic and T1 ribonucleases.来自啤酒酵母的tRNAIIArg的一级结构。1. 用胰核糖核酸酶和T1核糖核酸酶进行完全消化。
Eur J Biochem. 1975 Aug 15;56(2):521-6. doi: 10.1111/j.1432-1033.1975.tb02257.x.
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Yeast tRNA Leu UAG. Purification, properties and determination of the nucleotide sequence by radioactive derivative methods.酵母tRNA亮氨酸UAG。通过放射性衍生物方法进行纯化、性质研究及核苷酸序列测定。
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6
The primary structure of non-initiator methionine transfer ribonucleic acid from Bakers' yeast. I. Purification and complete digestion with ribonuclease T1 and pancreatic ribonuclease A.来自面包酵母的非起始甲硫氨酸转移核糖核酸的一级结构。I. 用核糖核酸酶T1和胰核糖核酸酶A进行纯化及完全消化。
J Biochem. 1976 Nov;80(5):937-50. doi: 10.1093/oxfordjournals.jbchem.a131381.
7
The nucleotide sequence of cysteine transfer ribonucleic acid from baker's yeast. Identification of the products from partial degradation of the molecule and derivation of the complete sequence.面包酵母半胱氨酸转移核糖核酸的核苷酸序列。该分子部分降解产物的鉴定及完整序列的推导。
Biochem J. 1976 Feb 1;153(2):447-54. doi: 10.1042/bj1530447.
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Investigation of recognition sites in valine tRNA I (Baker's yeast) by dissected molecule method.用切割分子法研究缬氨酸tRNA I（面包酵母）中的识别位点
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Nucleotide sequence of tRNA Cys from baker's yeast.来自面包酵母的tRNA半胱氨酸的核苷酸序列。
FEBS Lett. 1974 Sep 15;46(1):268-70. doi: 10.1016/0014-5793(74)80384-4.
10
The acceptor activity of dissected molecules of Baker's yeast tRNA Val 1 : localization of two possible recognition sites of valyl-tRNA ligase.面包酵母tRNA Val 1切割分子的受体活性：缬氨酰-tRNA连接酶两个可能识别位点的定位
Mol Biol. 1972 Jan-Feb;6(1):69-84.

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本文引用的文献

1
SPECIFIC CLEAVAGE OF THE YEAST ALANINE RNA INTO TWO LARGE FRAGMENTS.酵母丙氨酸RNA特异性切割成两个大片段。
Proc Natl Acad Sci U S A. 1965 Mar;53(3):543-8. doi: 10.1073/pnas.53.3.543.
2
[CHROMATOGRAPHIC MAPPING OF RIBONUCLEASE HYDROLYSATES OF TRANSFER RNA].[转移核糖核酸核糖核酸酶水解产物的色谱图谱分析]
Biokhimiia. 1964 Mar-Apr;29:333-7.
3
Methods for selective inactivation of phosphodiesterase and 5-nucleotidase of gurza (Vipera lebetina) venom.选择性灭活古尔扎（草原蝰）毒液磷酸二酯酶和5-核苷酸酶的方法。
含有尿苷而非核糖胸腺嘧啶的小麦胚芽转运RNA：一类异常真核转运RNA的特性
Nucleic Acids Res. 1978 Apr;5(4):1075-92. doi: 10.1093/nar/5.4.1075.
Biochim Biophys Acta. 1962 Oct 8;64:197-9. doi: 10.1016/0006-3002(62)90780-1.
4
The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose.在苯甲酰化二乙氨基乙基纤维素上分离可溶性核糖核酸。
Biochemistry. 1967 Oct;6(10):3043-56. doi: 10.1021/bi00862a011.
5
Separation of transfer ribonucleic acids by hydroxyapatite columns.用羟基磷灰石柱分离转移核糖核酸
J Biol Chem. 1966 Feb 10;241(3):767-9.
6
Chromatography of tri- and tetranucleotides from pancreatic ribonuclease digests of ribonucleic acid.核糖核酸经胰核糖核酸酶消化后产生的三核苷酸和四核苷酸的色谱分析。
Biochem Biophys Res Commun. 1964;14:276-9. doi: 10.1016/0006-291x(64)90449-8.
7
Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns.在聚氯三氟乙烯支持的反相色谱柱上改进转运RNA的分离。
Biochim Biophys Acta. 1971 Feb 11;228(3):770-4. doi: 10.1016/0005-2787(71)90748-9.
8
[Rapid method of separation and analysis of a mixture of oligonucleotides].[寡核苷酸混合物的快速分离与分析方法]
Bull Soc Chim Biol (Paris). 1970 Mar 31;52(2):125-33.
9
Nucleotide sequence of E. coli B tRNA1-Val.大肠杆菌B tRNA1-缬氨酸的核苷酸序列。
Nature. 1969 Apr 19;222(5190):278-9. doi: 10.1038/222278a0.
10
Nucleotide sequence of rabbit liver and sheep mammary gland cytoplasmic initiatory transfer RNAs.兔肝脏和绵羊乳腺细胞质起始转运核糖核酸的核苷酸序列。
Nature. 1974 Feb 22;247(5442):518-20. doi: 10.1038/247518a0.