Iwata H, Murase K, Inoue T
Department of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Japan.
J Vet Med Sci. 2000 Oct;62(10):1099-100. doi: 10.1292/jvms.62.1099.
Monoclonal antibodies (Mabs) against bovine alpha1-acid glycoprotein (alpha1AGP) were prepared from mouse hybridoma cell line. Bovine alpha1AGP as antigen was purified by using ion exchange column chromatography and the yield from 500 ml serum was about 10 mg. Immunoglobulin isotypes of 3 Mabs obtained were IgM and light chain types were kappa. The Mabs reacted with bovine alpha1AGP on immunoblot analysis, but not with alpha1AGP digested with N-glycosidase, suggesting that an epitope recognized by these Mabs may be associated with a glycan side chain of bovine alpha1AGP.
从小鼠杂交瘤细胞系制备了抗牛α1-酸性糖蛋白（α1AGP）的单克隆抗体（Mabs）。以牛α1AGP作为抗原，通过离子交换柱色谱法进行纯化，500ml血清的产量约为10mg。获得的3种单克隆抗体的免疫球蛋白同种型为IgM，轻链类型为κ。这些单克隆抗体在免疫印迹分析中与牛α1AGP反应，但不与用N-糖苷酶消化的α1AGP反应，这表明这些单克隆抗体识别的表位可能与牛α1AGP的聚糖侧链相关。
