特定RNA对真核生物翻译终止的抑制作用。
Suppression of eukaryotic translation termination by selected RNAs.
作者信息
Carnes J, Frolova L, Zinnen S, Drugeon G, Phillippe M, Justesen J, Haenni A L, Leinwand L, Kisselev L L, Yarus M
机构信息
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347, USA.
出版信息
RNA. 2000 Oct;6(10):1468-79. doi: 10.1017/s1355838200001242.
Using selection-amplification, we have isolated RNAs with affinity for translation termination factors eRF1 and eRF1.eRF3 complex. Individual RNAs not only bind, but inhibit eRF1-mediated release of a model nascent chain from eukaryotic ribosomes. There is also significant but weaker inhibition of eRF1-stimulated eRF3 GTPase and eRF3 stimulation of eRF1 release activity. These latter selected RNAs therefore hinder eRF1.eRF3 interactions. Finally, four RNA inhibitors of release suppress a UAG stop codon in mammalian extracts dependent for termination on eRF1 from several metazoan species. These RNAs are therefore new specific inhibitors for the analysis of eukaryotic termination, and potentially a new class of omnipotent termination suppressors with possible therapeutic significance.
通过选择扩增，我们分离出了对翻译终止因子eRF1和eRF1.eRF3复合物具有亲和力的RNA。单个RNA不仅能结合，还能抑制eRF1介导的模型新生链从真核核糖体上的释放。对eRF1刺激的eRF3 GTP酶活性以及eRF3对eRF1释放活性的刺激也有显著但较弱的抑制作用。因此，这些后来筛选出的RNA会阻碍eRF1.eRF3的相互作用。最后，四种释放抑制性RNA在依赖几种后生动物物种的eRF1进行终止的哺乳动物提取物中能抑制UAG终止密码子。因此，这些RNA是用于分析真核生物终止的新型特异性抑制剂，并且可能是一类具有潜在治疗意义的全能终止抑制因子。
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