大肠杆菌在苹果酸上依赖H2的厌氧生长：琥珀酸的形成
H2-dependent anaerobic growth of Escherichia coli on L-malate: succinate formation.
作者信息
Macy J, Kulla H, Gottschalk G
出版信息
J Bacteriol. 1976 Feb;125(2):423-8. doi: 10.1128/jb.125.2.423-428.1976.
Abstract
Escherichia coli grew anaerobically on L-malate only in the presence of H2; 91% of the L-malate utilized was converted to succinate. Anaerobically isolated membrane vesicles catalyzed the reduction of fumarate with H2 and contained a b-type cytochrome. Cytochrome c552 was present in the "periplasmic space."
摘要
大肠杆菌仅在有氢气存在的情况下才能以L-苹果酸为底物进行厌氧生长；所利用的L-苹果酸中有91%被转化为琥珀酸。厌氧分离得到的膜囊泡能催化氢气还原富马酸，并含有一种b型细胞色素。细胞色素c552存在于“周质空间”中。
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本文引用的文献
1
The role of fumarate in the respiration of Bacterium coli commune.延胡索酸在普通大肠杆菌呼吸作用中的作用。
Biochem J. 1937 Nov;31(11):2095-124. doi: 10.1042/bj0312095.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
BIOLOGICAL FORMATION OF MOLECULAR HYDROGEN.分子氢的生物形成
Science. 1965 Apr 9;148(3667):186-92. doi: 10.1126/science.148.3667.186.
4
A FUMARATE REDUCTASE IN ESCHERICHIA COLI DISTINCT FROM SUCCINATE DEHYDROGENASE.大肠杆菌中一种不同于琥珀酸脱氢酶的延胡索酸还原酶。
J Biol Chem. 1963 Nov;238:3770-4.
5
Electron-transport system of Vibrio succinogenes. II. Inhibition of electron transport by 2-heptyl-4-hydroxyquinoline N-oxide.产琥珀酸弧菌的电子传递系统。II. 2-庚基-4-羟基喹啉N-氧化物对电子传递的抑制作用。
Biochim Biophys Acta. 1963 Jan 1;69:29-39. doi: 10.1016/0006-3002(63)91222-8.
6
Electron-transport system of Vibrio succinogenes. I. Enzymes and cytochromes of electron-transport system.琥珀酸弧菌的电子传递系统。I. 电子传递系统的酶和细胞色素
Biochim Biophys Acta. 1963 Jan 1;69:18-28. doi: 10.1016/0006-3002(63)91221-6.
7
Cytochrome-producing anaerobic Vibrio succinogenes, sp. n.产细胞色素的厌氧琥珀酸弧菌，新种
J Bacteriol. 1961 Jun;81(6):911-7. doi: 10.1128/jb.81.6.911-917.1961.
8
Comparative biochemistry of the biological reduction of fumaric acid.富马酸生物还原的比较生物化学
Biochim Biophys Acta. 1957 Jul;25(1):142-7. doi: 10.1016/0006-3002(57)90431-6.
9
Phosphorylation coupled to oxidation of hydrogen with fumarate in extracts of the sulfate reducing bacterium, Desulfovibrio gigas.在硫酸盐还原菌巨大脱硫弧菌的提取物中，磷酸化与氢和富马酸酯的氧化相偶联。
Biochem Biophys Res Commun. 1970 Nov 25;41(4):1036-42. doi: 10.1016/0006-291x(70)90189-0.
10
Regulation of metabolism in facultative bacteria. I. Structural and functional changes in Escherichia coli associated with shifts between the aerobic and anaerobic states.兼性细菌的代谢调节。I. 与有氧和无氧状态转变相关的大肠杆菌的结构和功能变化。
Biochim Biophys Acta. 1966 Mar 28;117(1):22-32. doi: 10.1016/0304-4165(66)90148-6.
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