Goldberg I, Lonberg-Holm K, Bagley E A, Stieglitz B
Appl Environ Microbiol. 1983 Jun;45(6):1838-47. doi: 10.1128/aem.45.6.1838-1847.1983.
Two recombinant plasmid Escherichia coli strains containing amplified fumarate reductase activity converted fumarate to succinate at significantly higher rates and yields than a wild-type E. coli strain. Glucose was required for the conversion of fumarate to succinate, and in the absence of glucose or in cultures with a low cell density, malate accumulated. Two-dimensional gel electrophoretic analysis of proteins from the recombinant DNA and wild-type strains showed that increased quantities of both large and small fumarate reductase subunits were expressed in the recombinant DNA strains.
两株含有扩增富马酸还原酶活性的重组质粒大肠杆菌菌株将富马酸转化为琥珀酸的速率和产量明显高于野生型大肠杆菌菌株。富马酸转化为琥珀酸需要葡萄糖，在没有葡萄糖的情况下或在细胞密度低的培养物中，苹果酸会积累。对重组DNA菌株和野生型菌株的蛋白质进行二维凝胶电泳分析表明，重组DNA菌株中表达的大小富马酸还原酶亚基的量均增加。
