Ljunglöf A, Larsson M, Knuuttila K G, Lindgren J
Amersham Pharmacia Biotech, Uppsala, Sweden.
J Chromatogr A. 2000 Oct 6;893(2):235-44. doi: 10.1016/s0021-9673(00)00684-1.
Two methods, confocal scanning laser microscopy and confocal micro-Raman spectroscopy were used to analyse the distribution of IgG antibodies immobilized on CNBr-activated agarose beads. In the first method the internal distribution profile of fluorescent labelled Protein A was used as an indirect measure of the distribution of IgG, while the second method detects vibrations originating from aromatic amino acids present in the immobilized antibodies. Both these methods indicate an homogeneous ligand distribution within IgG Sepharose 4 Fast Flow and IgG Sepharose 6 Fast Flow.
使用共聚焦扫描激光显微镜和共聚焦显微拉曼光谱两种方法分析固定在溴化氰活化琼脂糖珠上的IgG抗体的分布。在第一种方法中，荧光标记的蛋白A的内部分布轮廓被用作IgG分布的间接测量指标，而第二种方法检测固定化抗体中存在的芳香族氨基酸产生的振动。这两种方法均表明IgG琼脂糖4快速流动和IgG琼脂糖6快速流动中的配体分布均匀。
