Field J A, Sawyer T E
Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis 97331, USA. jennifer.field@.orst.edu
J Chromatogr A. 2000 Oct 6;893(2):253-60. doi: 10.1016/s0021-9673(00)00764-0.
A method utilizing direct aqueous injection with high-performance liquid chromatography and diode array detection (HPLC-DAD) is presented for the quantitation determination of trichloroethene (TCE) in the presence of anionic surfactants that are used to enhance the recovery of dense non-aqueous phase liquids from contaminated groundwater aquifers. The anionic surfactants investigated in this study including alkyl diphenyloxide disulfonate (Dowfax 8390) and dihexylsulfosuccinate (Aerosol MA 80-1) are used to enhance the solubility, and hence recovery, of TCE. In this type of environmental engineering application, the levels of surfactants and TCE encountered are very high (part per million to part per thousand). The anionic surfactants and TCE are quantitatively determined by direct aqueous injection onto a reversed-phase HPLC column with diode array detection. The quantitation limits of the method obtained using 100 microl injections are 0.1 mg/l for alkyl diphenyloxide disulfonates, 20 mg/l for dihexylsulfosuccinate, and 0.05 mg/l for TCE. This approach is advantageous over using gas chromatography for TCE and HPLC for the surfactants because the use of a single analytical instrument reduces sample preparation and analysis times, which increases sample throughput.
本文介绍了一种利用直接水相进样结合高效液相色谱和二极管阵列检测（HPLC-DAD）的方法，用于在存在阴离子表面活性剂的情况下定量测定三氯乙烯（TCE）。这些阴离子表面活性剂用于提高从受污染的地下水含水层中回收致密非水相液体的效率。本研究中所研究的阴离子表面活性剂包括烷基二苯醚二磺酸盐（陶氏化学8390）和二己基磺基琥珀酸盐（气溶胶MA 80-1），它们用于提高TCE的溶解度，进而提高其回收率。在这类环境工程应用中，所遇到的表面活性剂和TCE的含量非常高（百万分之几到千分之几）。通过直接水相进样到配备二极管阵列检测的反相HPLC柱上，对阴离子表面活性剂和TCE进行定量测定。使用100微升进样量获得的该方法的定量限为：烷基二苯醚二磺酸盐为0.1毫克/升，二己基磺基琥珀酸盐为20毫克/升，TCE为0.05毫克/升。这种方法相对于使用气相色谱法测定TCE和高效液相色谱法测定表面活性剂具有优势，因为使用单一分析仪器可减少样品制备和分析时间，从而提高样品通量。
