Desportes C, Charpentier M, Duteurtre B, Maujean A, Duchiron F
Laboratoire d 'Oenologie, Université de Reims Champagne Ardennes, Moulin de la Housse, Reims, France.
J Chromatogr A. 2000 Oct 6;893(2):281-91. doi: 10.1016/s0021-9673(00)00698-1.
Peptides are difficult to isolate from wine because they are present in a complex mixture together with non-peptidic compounds. A method for the isolation, separation and purity assessing of small peptides is proposed. Small peptides (Mr<3000) were isolated from wine by hollow fibre ultrafiltration followed by column chromatography using the gel matrix Sephadex LH20. Fractions obtained by gel filtration on Sephadex LH20 were subjected to HPLC on a porous graphitic carbon column in order to isolate small peptides. Peak purity was then analysed by capillary electrophoresis.
肽类物质很难从葡萄酒中分离出来，因为它们与非肽类化合物一起存在于复杂的混合物中。本文提出了一种分离、纯化和评估小肽纯度的方法。通过中空纤维超滤从葡萄酒中分离出小肽（Mr<3000），然后使用凝胶基质Sephadex LH20进行柱色谱分离。将在Sephadex LH20上进行凝胶过滤得到的馏分在多孔石墨碳柱上进行HPLC分析，以分离小肽。然后通过毛细管电泳分析峰纯度。
