霍乱弧菌中的无机多聚磷酸盐：遗传、生化及生理特征
Inorganic polyphosphate in Vibrio cholerae: genetic, biochemical, and physiologic features.
作者信息
Ogawa N, Tzeng C M, Fraley C D, Kornberg A
机构信息
Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305-5307, USA.
出版信息
J Bacteriol. 2000 Dec;182(23):6687-93. doi: 10.1128/JB.182.23.6687-6693.2000.
Abstract
Vibrio cholerae O1, biotype El Tor, accumulates inorganic polyphosphate (poly P) principally as large clusters of granules. Poly P kinase (PPK), the enzyme that synthesizes poly P from ATP, is encoded by the ppk gene, which has been cloned from V. cholerae, overexpressed, and knocked out by insertion-deletion mutagenesis. The predicted amino acid sequence of PPK is 701 residues (81.6 kDa), with 64% identity to that of Escherichia coli, which it resembles biochemically. As in E. coli, ppk is part of an operon with ppx, the gene that encodes exopolyphosphatase (PPX). However, unlike in E. coli, PPX activity was not detected in cell extracts of wild-type V. cholerae. The ppk null mutant of V. cholerae has diminished adaptation to high concentrations of calcium in the medium as well as motility and abiotic surface attachment.
摘要
霍乱弧菌O1型，埃尔托生物型，主要以大颗粒簇的形式积累无机多聚磷酸盐（多聚P）。多聚P激酶（PPK）是一种由ATP合成多聚P的酶，由ppk基因编码，该基因已从霍乱弧菌中克隆、过表达，并通过插入缺失诱变被敲除。PPK的预测氨基酸序列为701个残基（81.6 kDa），与大肠杆菌的序列有64%的同源性，在生化性质上与之相似。与大肠杆菌一样，ppk是一个操纵子的一部分，该操纵子包含ppx，即编码外多聚磷酸酶（PPX）的基因。然而，与大肠杆菌不同的是，在野生型霍乱弧菌的细胞提取物中未检测到PPX活性。霍乱弧菌的ppk基因缺失突变体对培养基中高浓度钙的适应性、运动性和非生物表面附着能力均有所下降。
相似文献
1
Inorganic polyphosphate in Vibrio cholerae: genetic, biochemical, and physiologic features.
J Bacteriol. 2000 Dec;182(23):6687-93. doi: 10.1128/JB.182.23.6687-6693.2000.
2
Polyphosphate stores enhance the ability of Vibrio cholerae to overcome environmental stresses in a low-phosphate environment.
Appl Environ Microbiol. 2006 Nov;72(11):7043-9. doi: 10.1128/AEM.00924-06. Epub 2006 Sep 1.
3
Two-step processing for activation of the cytolysin/hemolysin of Vibrio cholerae O1 biotype El Tor: nucleotide sequence of the structural gene (hlyA) and characterization of the processed products.
Infect Immun. 1990 Dec;58(12):4106-16. doi: 10.1128/iai.58.12.4106-4116.1990.
4
Identification of the Vibrio cholerae type 4 prepilin peptidase required for cholera toxin secretion and pilus formation.
Mol Microbiol. 1998 Sep;29(6):1481-92. doi: 10.1046/j.1365-2958.1998.01031.x.
5
Cloning and sequencing of a novel hemolysis gene of Vibrio cholerae.
FEMS Microbiol Lett. 1995 May 15;128(3):265-9. doi: 10.1111/j.1574-6968.1995.tb07534.x.
6
Identification of the gene for the monomeric alkaline phosphatase of Vibrio cholerae serogroup O1 strain.
Gene. 2005 Jan 3;344:251-8. doi: 10.1016/j.gene.2004.11.005. Epub 2004 Dec 10.
7
TcpA pilin sequences and colonization requirements for O1 and O139 vibrio cholerae.
Mol Microbiol. 1994 Sep;13(6):1013-20. doi: 10.1111/j.1365-2958.1994.tb00492.x.
8
An exopolyphosphatase of Escherichia coli. The enzyme and its ppx gene in a polyphosphate operon.
J Biol Chem. 1993 Jan 5;268(1):633-9.
9
Characterization of the Vibrio cholerae El Tor lipase operon lipAB and a protease gene downstream of the hly region.
J Bacteriol. 1997 Nov;179(22):7072-80. doi: 10.1128/jb.179.22.7072-7080.1997.
10
Molecular characterization of polyphosphate (PolyP) operon from Serratia marcescens.
J Basic Microbiol. 2006;46(2):108-15. doi: 10.1002/jobm.200510038.
引用本文的文献
1
Inorganic polyphosphate and the stringent response coordinately control cell division and cell morphology in .
mBio. 2025 Feb 5;16(2):e0351124. doi: 10.1128/mbio.03511-24. Epub 2024 Dec 27.
2
kills in a polyphosphate-dependent manner.
mSphere. 2024 Oct 29;9(10):e0068624. doi: 10.1128/msphere.00686-24. Epub 2024 Oct 4.
3
Inorganic polyphosphate and the stringent response coordinately control cell division and cell morphology in .
bioRxiv. 2024 Sep 12:2024.09.11.612536. doi: 10.1101/2024.09.11.612536.
4
C-terminal Poly-histidine Tags Alter Escherichia coli Polyphosphate Kinase Activity and Susceptibility to Inhibition.
J Mol Biol. 2024 Aug 15;436(16):168651. doi: 10.1016/j.jmb.2024.168651. Epub 2024 Jun 10.
5
The Protein Scaffolding Functions of Polyphosphate.
J Mol Biol. 2024 Jul 15;436(14):168504. doi: 10.1016/j.jmb.2024.168504. Epub 2024 Feb 27.
6
Predicted metabolic roles and stress responses provide insights into candidate phyla Hydrogenedentota and Sumerlaeota as members of the rare biosphere in biofilms from various environments.
Environ Microbiol Rep. 2024 Feb;16(1):e13228. doi: 10.1111/1758-2229.13228. Epub 2024 Jan 9.
7
kills in a polyphosphate-dependent manner.
bioRxiv. 2023 Dec 6:2023.12.05.570291. doi: 10.1101/2023.12.05.570291.
8
Make or break: the thermodynamic equilibrium of polyphosphate kinase-catalysed reactions.
Beilstein J Org Chem. 2022 Sep 20;18:1278-1288. doi: 10.3762/bjoc.18.134. eCollection 2022.
9
Inorganic polyphosphate in host and microbe biology.
Trends Microbiol. 2021 Nov;29(11):1013-1023. doi: 10.1016/j.tim.2021.02.002. Epub 2021 Feb 22.
10
Functional Insights Into the Role of in (p)ppGpp Metabolism of .
Front Microbiol. 2020 Sep 29;11:564644. doi: 10.3389/fmicb.2020.564644. eCollection 2020.
本文引用的文献
1
New components of a system for phosphate accumulation and polyphosphate metabolism in Saccharomyces cerevisiae revealed by genomic expression analysis.
Mol Biol Cell. 2000 Dec;11(12):4309-21. doi: 10.1091/mbc.11.12.4309.
2
Polyphosphate kinase is essential for biofilm development, quorum sensing, and virulence of Pseudomonas aeruginosa.
Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9636-41. doi: 10.1073/pnas.170283397.
3
Inorganic polyphosphate is needed for swimming, swarming, and twitching motilities of Pseudomonas aeruginosa.
Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4885-90. doi: 10.1073/pnas.060030097.
4
The multiple activities of polyphosphate kinase of Escherichia coli and their subunit structure determined by radiation target analysis.
J Biol Chem. 2000 Feb 11;275(6):3977-83. doi: 10.1074/jbc.275.6.3977.
5
Inorganic polyphosphate is required for motility of bacterial pathogens.
J Bacteriol. 2000 Jan;182(1):225-7. doi: 10.1128/JB.182.1.225-227.2000.
6
Inorganic polyphosphate kinase is required to stimulate protein degradation and for adaptation to amino acid starvation in Escherichia coli.
Proc Natl Acad Sci U S A. 1999 Dec 7;96(25):14264-9. doi: 10.1073/pnas.96.25.14264.
7
Inorganic polyphosphate: a molecule of many functions.
Prog Mol Subcell Biol. 1999;23:1-18. doi: 10.1007/978-3-642-58444-2_1.
8
Vibrio cholerae O1 El Tor: identification of a gene cluster required for the rugose colony type, exopolysaccharide production, chlorine resistance, and biofilm formation.
Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):4028-33. doi: 10.1073/pnas.96.7.4028.
9
Polyphosphate kinase is highly conserved in many bacterial pathogens.
Mol Microbiol. 1998 Jul;29(1):381-2. doi: 10.1046/j.1365-2958.1998.00887.x.
10
Inorganic polyphosphate in Escherichia coli: the phosphate regulon and the stringent response.
J Bacteriol. 1998 Apr;180(8):2186-93. doi: 10.1128/JB.180.8.2186-2193.1998.
Zotero 插件