霍乱弧菌O1 El Tor：鉴定形成皱缩菌落类型、产生胞外多糖、耐氯和形成生物膜所需的基因簇。
Vibrio cholerae O1 El Tor: identification of a gene cluster required for the rugose colony type, exopolysaccharide production, chlorine resistance, and biofilm formation.
作者信息
Yildiz F H, Schoolnik G K
机构信息
Department of Medicine, Division of Infectious Diseases and Geographic Medicine, and Department of Microbiology and Immunology, Stanford University Medical School, Beckman Center, Room 239, Stanford, CA 94305, USA.
出版信息
Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):4028-33. doi: 10.1073/pnas.96.7.4028.
Abstract
The rugose colony variant of Vibrio cholerae O1, biotype El Tor, is shown to produce an exopolysaccharide, EPSETr, that confers chlorine resistance and biofilm-forming capacity. EPSETr production requires a chromosomal locus, vps, that contains sequences homologous to carbohydrate biosynthesis genes of other bacterial species. Mutations within this locus yield chlorine-sensitive, smooth colony variants that are biofilm deficient. The biofilm-forming properties of EPSETr may enable the survival of V. cholerae O1 within environmental aquatic habitats between outbreaks of human disease.
摘要
霍乱弧菌O1型埃尔托生物型的粗糙菌落变体被证明能产生一种胞外多糖EPSETr，它赋予了对氯的抗性和生物膜形成能力。EPSETr的产生需要一个染色体位点vps，该位点包含与其他细菌物种的碳水化合物生物合成基因同源的序列。该位点内的突变会产生对氯敏感的光滑菌落变体，这些变体缺乏生物膜形成能力。EPSETr的生物膜形成特性可能使霍乱弧菌O1型在人类疾病爆发之间的环境水生栖息地中存活下来。
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