用于细菌学质量控制目的的模拟水样制备。
The preparation of simulated water samples for the purpose of bacteriological quality control.
作者信息
Gray R D, Lowe G H
出版信息
J Hyg (Lond). 1976 Feb;76(1):49-56. doi: 10.1017/s0022172400054930.
As part of the bacteriological quality control programme of the Public Health Laboratory Service, we were asked to investigate the possibility of providing simulated water samples for distribution to the laboratories. For this purpose it was necessary to find some means whereby suspensions of coliform organisms and Escherichia coli could be kept relatively stable in number at room temperature for a period of 7-10 days. This, it was finally found, was best achieved by adding selected strains of the organisms to improved formate lactose glutamate medium (Gray, 1964) without the lactose but with added boric acid to a final concentration of 1-8%. The procedures adopted in the successful quality control programme are described.
作为公共卫生实验室服务细菌学质量控制计划的一部分，我们被要求研究提供模拟水样分发给各实验室的可能性。为此，有必要找到某种方法，使大肠菌群和大肠杆菌的悬浮液在室温下数量能相对稳定地保持7至10天。最终发现，将选定的菌株添加到改良的甲酸盐乳糖谷氨酸培养基（格雷，1964年）中可最好地实现这一点，该培养基不含乳糖，但添加了硼酸，最终浓度为1 - 8%。本文描述了成功的质量控制计划所采用的程序。
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