Uszynski M, Klyszejko A, Zekanowska E
Department of Medical Propedeutics, Ludwik Rydygier Medical University ul. M. Curie-Sklodowskiej 9, 85 067 Bydgoszcz, Poland.
Eur J Obstet Gynecol Reprod Biol. 2000 Dec;93(2):167-71. doi: 10.1016/s0301-2115(00)00283-9.
The aim of the present study was to determine whether the following components of the fibrinolytic system are present in amniotic fluid: plasminogen, plasmin and alpha(2)-antiplasmin, and plasmin-alpha(2)-antiplasmin (PAP) complexes. The study group consisted of 30 healthy women giving birth at term; 10 nonpregnant women constituted a control group. Plasminogen and alpha(2)-antiplasmin were studied by using an amidolytic method, and PAP by an ELISA method. The levels of plasminogen and alpha(2)-antiplasmin in amniotic fluid are low, 1.57+/-0.82 and 6.80+/-3.09%, respectively, of the values in blood plasma. The PAP concentration in amniotic fluid (70.61+/-14.28 microg/l) was also lower than in blood plasma (247.19+/-111.39 microg/l). We concluded that in amniotic fluid plasminogen and alpha(2)-antiplasmin are present, but at a concentration lower than in plasma. The presence of PAP in amniotic fluid is an evidence of two processes in the amniotic cavity: (i) plasmin generation, and (ii) plasmin inactivation by alpha(2)-antiplasmin.
纤溶酶原、纤溶酶和α2-抗纤溶酶，以及纤溶酶-α2-抗纤溶酶（PAP）复合物。研究组由30名足月分娩的健康女性组成；10名未怀孕女性构成对照组。采用酰胺水解法研究纤溶酶原和α2-抗纤溶酶，采用ELISA法研究PAP。羊水中纤溶酶原和α2-抗纤溶酶的水平较低，分别为血浆中值的1.57±0.82%和6.80±3.09%。羊水中PAP浓度（70.61±14.28μg/l）也低于血浆（247.19±111.39μg/l）。我们得出结论，羊水中存在纤溶酶原和α2-抗纤溶酶，但其浓度低于血浆。羊水中PAP的存在证明了羊膜腔内的两个过程：（i）纤溶酶的产生，以及（ii）α2-抗纤溶酶对纤溶酶的失活作用。
