Abel E A, Bystryn J C
J Invest Dermatol. 1976 Feb;66(02):117-21. doi: 10.1111/1523-1747.ep12481466.
The reproducibility of the indirect immunofluorescent test for antibodies to human malignant melanoma cytoplasmic antigens was investigated by testing panels of melanoma and normal sera against cells of each of several different melanomas. Tests were performed in replicate, read blindly by two observers, and repeated on different days. Different observers agreed in the interpretation of replicate assays in approximately 80 to 90% of cases. Variability increased considerably when assays were repeated on different days or when different in as few as 21% of tests. Thus, the results of the indirect immunofluorescent test for melanoma cytoplasmic antibodies must, at the present time, be interpreted with great caution.
通过使用黑色素瘤和正常血清组检测几种不同黑色素瘤细胞，研究了人恶性黑色素瘤细胞质抗原抗体间接免疫荧光试验的可重复性。试验进行了重复检测，由两名观察者盲法读取结果，并在不同日期重复进行。不同观察者在约80%至90%的病例中对重复检测结果的解释一致。当在不同日期重复检测或检测不同细胞系时，变异性显著增加，在少至21%的检测中也是如此。因此，目前对黑色素瘤细胞质抗体间接免疫荧光试验的结果必须极其谨慎地解释。
