一种新型钴 - 白蛋白结合测定法及其作为心肌缺血标志物的潜力——初步报告。

A novel assay for cobalt-albumin binding and its potential as a marker for myocardial ischemia-a preliminary report.

作者信息

Bar-Or D, Lau E, Winkler J V

机构信息

Department of Trauma Research, Swedish Medical Center, Englewood, Colorado 80110, USA.

出版信息

J Emerg Med. 2000 Nov;19(4):311-5. doi: 10.1016/s0736-4679(00)00255-9.

DOI:10.1016/s0736-4679(00)00255-9

PMID:11074321

Abstract

We initially observed a phenomenon of reduced in vitro binding of exogenous cobalt [Co(II)] to the N-terminus of human serum albumin (HSA) in emergency chest pain patients with early onset unstable angina and myocardial infarction. We then developed a colorimetric assay to measure cobalt-HSA binding and record the results in absorbance units (ABSU). In a preliminary clinical study of 139 emergency patients with acute chest pain, 99 patients with evidence of myocardial ischemia (Group 1) had elevated assay levels (mean ABSU +/- SD; 0.519 +/- 0.086) compared to 40 patients (Group 2) with no evidence of ischemia (0.316 +/- 0.092) (p < 0.00001). In Group 1, 95 of 99 (96.0%) patients had levels higher than a decision threshold of 0.400 ABSU and in Group 2, 37 of 40 (92.5%) samples had higher cobalt binding capacity (ABSU </= 0.400). Further studies are warranted to determine if an assay measuring altered cobalt-HSA binding is a clinically useful diagnostic test to rule out myocardial ischemia.

摘要

我们最初观察到，在患有早发不稳定型心绞痛和心肌梗死的急诊胸痛患者中，外源性钴[Co(II)]与人血清白蛋白（HSA）N端的体外结合减少。然后，我们开发了一种比色测定法来测量钴与HSA的结合，并以吸光度单位（ABSU）记录结果。在一项对139例急性胸痛急诊患者的初步临床研究中，99例有心肌缺血证据的患者（第1组）的测定水平升高（平均ABSU +/-标准差；0.519 +/- 0.086），而40例无缺血证据的患者（第2组）为（0.316 +/- 0.092）（p < 0.000,01）。在第1组中，99例患者中有95例（96.0%）的水平高于0.400 ABSU的判定阈值，在第2组中，40个样本中有37个（92.5%）具有较高的钴结合能力（ABSU <= 0.400）。有必要进行进一步研究，以确定测量钴与HSA结合改变的测定法是否是排除心肌缺血的临床有用诊断试验。