Hasturk Denizcan, Akturk Esen Selin, Sahiner Enes Seyda, Erel Ozcan, Neselioglu Salim, Aydogan Nurullah, Buyukaksoy Muge, Seven Ismet, Yucel Sebnem, Uncu Dogan
Clinic of Internal Medicine, Ankara Bilkent City Hospital, 06800, Ankara, Turkey.
Clinic of Medical Oncology, Ankara Bilkent City Hospital, 06800, Ankara, Turkey.
Sci Rep. 2025 Jun 5;15(1):19729. doi: 10.1038/s41598-025-04608-x.
In our study, we determined the changes in the oxidative stress (OxS) biomarkers, thiol-disulfide (TD) homeostasis and ischemia-modified albumin (IMA) levels, in patients diagnosed with ovarian carcinoma before and after chemotherapy. We will examine the indirect effects of chemotherapy on OxS and antioxidant capacity by measuring changes in these blood biomarkers and compare the results with those of the healthy control group. This case-control study, which was conducted in a single-center, prospective design, included 42 patients diagnosed with ovarian cancer and 51 healthy volunteers. Venous blood samples were taken from all participants after 8 h of fasting, their serum was separated, and the serum total thiol, native thiol, disulfide, and IMA values were measured. In the comparison of the blood samples taken before the chemotherapy treatment of the patient group with the healthy control group, the native thiol (p < 0.001), total thiol (p < 0.001), and native thiol/total thiol (p < 0.001) values were found to be statistically significantly lower, and the disulfide (p = 0.001), disulfide/native (p < 0.001), and disulfide/total thiol (p < 0.001) levels were found to be statistically significantly greater. In the patient group diagnosed with ovarian cancer, a statistically significant difference was detected between the measurements of cancer antigen-125 (CA-125) (p < 0.001), native thiol (p = 0.019), and total thiol (p = 0.025) values at the 0th month before chemotherapy treatment and the third month after chemotherapy treatment. In the group that received adjuvant chemotherapy after the operation, the native thiol (p = 0.035), total thiol (p = 0.043), disulfide/native thiol (p = 0.035), disulfide total thiol (p = 0.035), native thiol/total thiol (p = 0.035) and IMA (p = 0.026) values were statistically significantly different between the diagnosis and third-month values. Our study suggests that TD homeostasis may be an important guide in terms of disease progression, complications during chemotherapy treatment, appropriate dose reductions, and modifications in chemotherapy depending on the toxicities experienced and the goals of the treatment.
在我们的研究中，我们测定了经化疗的卵巢癌患者化疗前后氧化应激（OxS）生物标志物、硫醇 - 二硫键（TD）稳态及缺血修饰白蛋白（IMA）水平的变化。我们将通过测量这些血液生物标志物的变化来研究化疗对OxS和抗氧化能力的间接影响，并将结果与健康对照组进行比较。这项单中心前瞻性设计的病例对照研究纳入了42例诊断为卵巢癌的患者和51名健康志愿者。所有参与者在禁食8小时后采集静脉血样，分离血清，并测量血清总硫醇、天然硫醇、二硫键和IMA值。在将患者组化疗前采集的血样与健康对照组进行比较时，发现天然硫醇（p < 0.001）、总硫醇（p < 0.001）和天然硫醇/总硫醇（p < 0.001）值在统计学上显著较低，而二硫键（p = 0.001）、二硫键/天然硫醇（p < 0.001）和二硫键/总硫醇（p < 0.001）水平在统计学上显著较高。在诊断为卵巢癌的患者组中，化疗前第0个月与化疗后第3个月的癌抗原125（CA - 125）（p < 0.001）、天然硫醇（p = 0.019）和总硫醇（p = 0.025）值测量之间存在统计学显著差异。在术后接受辅助化疗的组中，诊断时与第3个月时的天然硫醇（p = 0.035）、总硫醇（p = 0.043）、二硫键/天然硫醇（p = 0.035）、二硫键/总硫醇（p = 0.035）、天然硫醇/总硫醇（p = 0.035）和IMA（p = 0.026）值在统计学上有显著差异。我们的研究表明，TD稳态在疾病进展、化疗治疗期间的并发症、适当的剂量减少以及根据所经历的毒性和治疗目标调整化疗方面可能是一个重要的指导。
