Dubinina E E, Morozova M G, Leonova N V, Gamper N L, Soliternova I B, Nuller Iu L, Butoma G B, Kovrugina S V
Department of Biochemistry, Psychoneurological Bekhterev Institute, St. Petersburg, Russia.
Vopr Med Khim. 2000 Jul-Aug;46(4):398-409.
We determined the oxidative modification of proteins (spontaneous and metal-catalysing oxidation, MKO) and the level of corticosteroids in patients with the depersonalization and depression. For detecting oxidative modification of plasma proteins we measured the concentration of protein carbonyl groups formed with 2,4dinitrophenylhydrazine 2,4dinitrophenylhydrazone derivatives; the formation of dityrosine by fluorescence method; protein aggregation and fragmentation. Polyacrylamide gel electrophoresis with sodium dodecyl sulfate (SDS)-PAGE in the presence beta-mercaptoethanol was used to determine the aggregation or fragmentation of proteins by oxygen radicals (OH). Acid-soluble peptides were analised as products of the fragmentation oxidative modification proteins. The level of the corticosteroids was determined using HPLS. The increase of the concentration of protein carbonyl groups in blood plasma of patients with mental disorders. In patients with depersonalization we determined the increase of the bityrosyl cross-link, and different degrees of fragmentation compared with depressive patients. The cortisol level was decreased and corticosterone was increased in the blood plasma of patients with depersonalization. In depressive patients the cortisol level was increased and corticosterone was decreased is discussed. We discussed the role oxidative modification proteins in the disturbance of the corticosteroid and opioid receptors functions in the patients with mental disorders.
我们测定了人格解体和抑郁症患者蛋白质的氧化修饰（自发氧化和金属催化氧化，MKO）以及皮质类固醇水平。为检测血浆蛋白质的氧化修饰，我们测量了用2,4-二硝基苯肼形成的2,4-二硝基苯腙衍生物中蛋白质羰基的浓度；通过荧光法测定二酪氨酸的形成；蛋白质聚集和片段化。在β-巯基乙醇存在下，采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）来确定氧自由基（OH）引起的蛋白质聚集或片段化。将酸溶性肽作为片段化氧化修饰蛋白质的产物进行分析。使用高效液相色谱法（HPLS）测定皮质类固醇水平。精神障碍患者血浆中蛋白质羰基浓度升高。在人格解体患者中，我们确定了二酪氨酸交联增加，且与抑郁症患者相比存在不同程度的片段化。人格解体患者血浆中皮质醇水平降低，皮质酮水平升高。讨论了抑郁症患者血浆中皮质醇水平升高而皮质酮水平降低的情况。我们讨论了氧化修饰蛋白质在精神障碍患者皮质类固醇和阿片受体功能紊乱中的作用。
