Tetik Sermin, Kiliç Arzu, Aksoy Halil, Rizaner Nahit, Ahmad Sarfraz, Yardimci Turay
Indian J Exp Biol. 2015 Jan;53(1):25-30.
We investigated the effect of oxidative systems on plasma proteins using Chloramine-T, a source of free radicals. Plasma specimens from 10 healthy volunteers were treated with 40 mmol/L Chloramine-T (1:1 v/v). Total protein and plasma carbonyl levels were evaluated spectrophotometrically. Identification of plasma proteins modifications was performed by SDS-PAGE, protein and lipid electrophoresis. Protein fragmentation was evaluated by HPLC. Total protein levels of oxidised plasmas were significantly lower (4.08 ± 0.12 g/dL) than control (7.86 ± 0.03 g/dL) (P < 0.01). Plasma carbonyl levels were higher (1.94 ± 0.38 nmol/mg protein) in oxidised plasma than that of control (0.03 ± 0.01 nmol/mg protein) (P < 0.01). Plasma oxidation had no significant effect on the levels of proteins and lipids. Protein fragmentations were detected in oxidised groups compared to those of the control. We conclude that protein modifications have direct effect on the protein functions, which are related to stress agent, its treatment period(s), and the methodology used for evaluating such experimental results.
我们使用自由基来源氯胺 - T研究了氧化系统对血浆蛋白的影响。来自10名健康志愿者的血浆样本用40 mmol/L氯胺 - T（1:1 v/v）处理。通过分光光度法评估总蛋白和血浆羰基水平。通过SDS - PAGE、蛋白质和脂质电泳对血浆蛋白修饰进行鉴定。通过HPLC评估蛋白质片段化。氧化血浆的总蛋白水平（4.08±0.12 g/dL）显著低于对照组（7.86±0.03 g/dL）（P<0.01）。氧化血浆中的血浆羰基水平（1.94±0.38 nmol/mg蛋白）高于对照组（0.03±0.01 nmol/mg蛋白）（P<0.01）。血浆氧化对蛋白质和脂质水平没有显著影响。与对照组相比，在氧化组中检测到蛋白质片段化。我们得出结论，蛋白质修饰对蛋白质功能有直接影响，这与应激剂、其处理时间以及用于评估此类实验结果的方法有关。
