[运用VIDAS-ELFA方法测定孕期抗弓形虫IgM]
[Dating anti-Toxoplasma IgM in pregnancy using VIDAS-ELFA methods].
作者信息
Pujol-Riqué M, Quintó L, Danés C, Valls M E, Coll O, Moreno A, Jiménez de Anta M T
机构信息
Servicio de Microbiología, Fundació Clínic, Barcelona.
出版信息
Enferm Infecc Microbiol Clin. 2000 Jun-Jul;18(6):274-8.
BACKGROUND
To study the usefulness of IgG and IgM titration, and avidity of IgG to date IgM anti-Toxoplasma gondii.
METHODS
VIDAS Toxo IgG, VIDAS Toxo IgM and VIDAS Toxo IgG Avidity tests were used. 64 sera containing both IgM and IgG T. gondii antibodies were analyzed, 32 from 12 individuals infected 40 weeks previously (group I), and the remainder from 17 individuals with an infection of more than 40 weeks (group II).
RESULTS
An IgM index < 1.05 was associated with an infection > 12 weeks. An avidity index > 0.164 excluded 100% infections of < or = 12 weeks. Avidity indexes > 0.26 and 0.45 excluded infections of < or = 20 and < or = 40 weeks, respectively.
CONCLUSIONS
The serology methods used in this study can adequately identify residual IgM anti-T. gondii, often avoiding the need of a new blood extraction to analyze IgG kinetics in pregnant women.
背景
研究IgG和IgM滴度测定以及IgG亲和力在弓形虫IgM抗体检测中的应用。
方法
采用VIDAS弓形虫IgG、VIDAS弓形虫IgM和VIDAS弓形虫IgG亲和力检测方法。分析了64份同时含有弓形虫IgM和IgG抗体的血清，其中32份来自40周前感染的12名个体（第一组），其余来自感染超过40周的17名个体（第二组）。
结果
IgM指数<1.05与感染超过12周相关。亲和力指数>0.164可100%排除感染12周及以内的情况。亲和力指数>0.26和>0.45分别可排除感染20周及以内和40周及以内的情况。
结论
本研究中使用的血清学方法能够充分识别残留的弓形虫IgM抗体，通常无需再次采血来分析孕妇的IgG动力学。
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